Overview

  • Product name
  • Description
    Rabbit polyclonal to SSTR4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human SSTR4 aa 366-388 (C terminal).
    Sequence:

    (C)QQEALQPEPGRKRIPLTRTTTF

  • Positive control
    • ICC/IF: SKNSH, NIH-3T3 and SH-SY5Y cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab28578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2500.
WB 1/100 - 1/5000. Detects a band of approximately 40-50 kDa (predicted molecular weight: 42 kDa).
ICC/IF 1/10 - 1/200.

Target

  • Function
    Receptor for somatostatin-14. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. It is functionally coupled not only to inhibition of adenylate cyclase, but also to activation of both arachidonate release and mitogen-activated protein (MAP) kinase cascade. Mediates antiproliferative action of somatostatin in tumor cells.
  • Tissue specificity
    Specifically expressed in fetal and adult brain, lung tissue, stomach, and in lesser quantities in the kidney, pituitary and adrenals.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Somatostatin Receptor 4 antibody
    • Somatostatin receptor type 4 antibody
    • SS 4R antibody
    • SS-4-R antibody
    • SS4-R antibody
    • SS4R antibody
    • SSR4_HUMAN antibody
    • SST R4 antibody
    • SSTR 4 antibody
    • Sstr4 antibody
    see all

Images

  • Immunofluorescent analysis of SSTR4 (green) showing staining in the cytoplasm of SH-SY5Y cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a ab28578 in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunofluorescent analysis of SSTR4 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab285781 in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • ICC/IF image of ab28578 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28578, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

References

ab28578 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us. ab28578 has been documented in IHC-P applications with human pheochromocytoma and human gastric mucosa sections. SSTR4 has been reported to be expressed in human brain, gastrointestinal tract, pancreas, and prostate tis...

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