Anti-SSX2 antibody [OTI4A11] (ab236415)

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Overview

  • Product name

    Anti-SSX2 antibody [OTI4A11]
    See all SSX2 primary antibodies

  • Description

    Mouse monoclonal [OTI4A11] to SSX2

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Human SSX2. (Expressed in HEK-293T cells).
    Database link: Q16385

  • Positive control

    • WB: HEK-293T cell lysate transfected with pCMV6-ENTRY SSX2 cDNA. IHC-P: Human breast adenocarcinoma, colon, colon adenocarcinoma, ovary, ovary adenocarcinoma, endometrium, prostate and bladder carcinoma tissue. ICC/IF: COS7 cells transiently transfected with pCMV6-ENTRY SSX2.

  • General notes

    Clone OTI4A11 (formerly 4A11).

Properties

Applications

Our Abpromise guarantee covers the use of ab236415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 22 kDa.
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100.

Target

  • Function

    Could act as a modulator of transcription.

  • Tissue specificity

    Expressed at high level in the testis. Expressed at low level in thyroid. Not detected in tonsil, colon, lung, spleen, prostate, kidney, striated and smooth muscles. Detected in rhabdomyosarcoma and fibrosarcoma cell lines. Not detected in mesenchymal and epithelial cell lines.

  • Involvement in disease

    Note=A chromosomal aberration involving SSX2 may be a cause of synovial sarcoma. Translocation t(X;18)(p11.2;q11.2). The translocation is specifically found in more than 80% of synovial sarcoma. The fusion products SSXT-SSX1 or SSXT-SSX2 are probably responsible for transforming activity. Heterogeneity in the position of the breakpoint can occur (low frequency).

  • Sequence similarities

    Belongs to the SSX family.
    Contains 1 KRAB-related domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Cancer/testis antigen 5.2 antibody
      • Cancer/testis antigen family 5, member 2a antibody
      • Cancer/testis antigen family 5, member 2b antibody
      • CT5.2 antibody
      • CT5.2A antibody
      • CT5.2b antibody
      • HD21 antibody
      • hom mel 40 antibody
      • HOM-MEL-40 antibody
      • MGC119055 antibody
      • MGC15364 antibody
      • MGC3884 antibody
      • Protein SSX2 antibody
      • RP11-552J9.2 antibody
      • sarcoma, synovial, X-chromosome related 2 antibody
      • SSx antibody
      • SSX2 antibody
      • SSX2-SYT fusion gene, included antibody
      • SSX2_HUMAN antibody
      • SSX2B antibody
      • Synovial sarcoma antibody
      • synovial sarcoma X breakpoint 2 antibody
      • Synovial sarcoma, X breakpoint 2B antibody
      • Tumor antigen HOM-MEL-40 antibody
      • X breakpoint 2 antibody
      see all

    Images

    • Western blot - Anti-SSX2 antibody [OTI4A11] (ab236415)
      Western blot - Anti-SSX2 antibody [OTI4A11] (ab236415)
      All lanes : Anti-SSX2 antibody [OTI4A11] (ab236415) at 1/2000 dilution

      Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
      Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY SSX2 cDNA

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 22 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)

      Paraffin-embedded human breast adenocarcinoma tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)

      Paraffin-embedded human colon tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)

      Paraffin-embedded human colon adenocarcinoma tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)

      Paraffin-embedded human ovary tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)

      Paraffin-embedded human ovary adenocarcinoma tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)

      Paraffin-embedded human endometrium tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)

      Paraffin-embedded human prostate tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)

      Paraffin-embedded human bladder carcinoma tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.

    • Immunocytochemistry/ Immunofluorescence - Anti-SSX2 antibody [OTI4A11] (ab236415)
      Immunocytochemistry/ Immunofluorescence - Anti-SSX2 antibody [OTI4A11] (ab236415)

      COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY SSX2 stained for SSX2 (green) using ab236415 at 1/100 dilution in ICC/IF.

    References

    ab236415 has not yet been referenced specifically in any publications.

    Publishing research using ab236415? Please let us know so that we can cite the reference in this datasheet.

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