Anti-SSX2 antibody [OTI4A11] (ab236415)
![Western blot - Anti-SSX2 antibody [OTI4A11] (ab236415)](https://www.abcam.com/ps/products/236/ab236415/Images/ab236415-312725-anti-ssx2-antibody-oti4a11-western-blot.jpg "Western blot - Anti-SSX2 antibody [OTI4A11] (ab236415)")
Key features and details
- Mouse monoclonal [OTI4A11] to SSX2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-SSX2 antibody [OTI4A11]
See all SSX2 primary antibodies -
Description
Mouse monoclonal [OTI4A11] to SSX2 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human SSX2. (Expressed in HEK-293T cells).
Database link: Q16385 -
Positive control
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY SSX2 cDNA. IHC-P: Human breast adenocarcinoma, colon, colon adenocarcinoma, ovary, ovary adenocarcinoma, endometrium, prostate and bladder carcinoma tissue. ICC/IF: COS7 cells transiently transfected with pCMV6-ENTRY SSX2.
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General notes
Clone OTI4A11 (formerly 4A11).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI4A11 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab236415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000. Predicted molecular weight: 22 kDa. | |
| IHC-P | 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100. |
Target
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Function
Could act as a modulator of transcription. -
Tissue specificity
Expressed at high level in the testis. Expressed at low level in thyroid. Not detected in tonsil, colon, lung, spleen, prostate, kidney, striated and smooth muscles. Detected in rhabdomyosarcoma and fibrosarcoma cell lines. Not detected in mesenchymal and epithelial cell lines. -
Involvement in disease
Note=A chromosomal aberration involving SSX2 may be a cause of synovial sarcoma. Translocation t(X;18)(p11.2;q11.2). The translocation is specifically found in more than 80% of synovial sarcoma. The fusion products SSXT-SSX1 or SSXT-SSX2 are probably responsible for transforming activity. Heterogeneity in the position of the breakpoint can occur (low frequency). -
Sequence similarities
Belongs to the SSX family.
Contains 1 KRAB-related domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6757 Human
- Entrez Gene: 727837 Human
- Omim: 300192 Human
- SwissProt: Q16385 Human
- Unigene: 289105 Human
- Unigene: 661107 Human
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Alternative names
- Cancer/testis antigen 5.2 antibody
- Cancer/testis antigen family 5, member 2a antibody
- Cancer/testis antigen family 5, member 2b antibody
see all
Images
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Western blot - Anti-SSX2 antibody [OTI4A11] (ab236415)All lanes : Anti-SSX2 antibody [OTI4A11] (ab236415) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY SSX2 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 22 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)](https://www.abcam.com/ps/products/236/ab236415/Images/ab236415-312726-anti-ssx2-antibody-oti4a11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)Paraffin-embedded human breast adenocarcinoma tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)](https://www.abcam.com/ps/products/236/ab236415/Images/ab236415-312729-anti-ssx2-antibody-oti4a11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)Paraffin-embedded human colon tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)](https://www.abcam.com/ps/products/236/ab236415/Images/ab236415-312730-anti-ssx2-antibody-oti4a11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)Paraffin-embedded human colon adenocarcinoma tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)](https://www.abcam.com/ps/products/236/ab236415/Images/ab236415-312731-anti-ssx2-antibody-oti4a11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)Paraffin-embedded human ovary tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)](https://www.abcam.com/ps/products/236/ab236415/Images/ab236415-312732-anti-ssx2-antibody-oti4a11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)Paraffin-embedded human ovary adenocarcinoma tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)](https://www.abcam.com/ps/products/236/ab236415/Images/ab236415-312733-anti-ssx2-antibody-oti4a11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)Paraffin-embedded human endometrium tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)](https://www.abcam.com/ps/products/236/ab236415/Images/ab236415-312734-anti-ssx2-antibody-oti4a11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)Paraffin-embedded human prostate tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)](https://www.abcam.com/ps/products/236/ab236415/Images/ab236415-312735-anti-ssx2-antibody-oti4a11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)Paraffin-embedded human bladder carcinoma tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.
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![Immunocytochemistry/ Immunofluorescence - Anti-SSX2 antibody [OTI4A11] (ab236415)](https://www.abcam.com/ps/products/236/ab236415/Images/ab236415-312736-anti-ssx2-antibody-oti4a11-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SSX2 antibody [OTI4A11] (ab236415)COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY SSX2 stained for SSX2 (green) using ab236415 at 1/100 dilution in ICC/IF.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab236415 has not yet been referenced specifically in any publications.
