Overview

  • Product name

    Anti-SSX2IP antibody [EPR16979]
    See all SSX2IP primary antibodies
  • Description

    Rabbit monoclonal [EPR16979] to SSX2IP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SSX2IP aa 600 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9Y2D8

  • Positive control

    • WB: HeLa, HEK293 and Jurkat whole cell lysates. Human testis lysates. IHC-P: Human cerebral cortex and human kidney tissue. IP: HEK293 whole cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab199425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/150.
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/10000. Detects a band of approximately 68, 71 kDa (predicted molecular weight: 68, 71 kDa).
ICC/IF 1/400.

Target

  • Function

    Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E-cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin.
  • Tissue specificity

    Widely expressed, with the highest expression in brain, intermediate expression in kidney, testis, spinal cord, liver, heart, lung, skeletal muscle, ovary, fetal liver and fetal brain, and little to no expression in pancreas and spleen. All specific brain regions showed intermediate to high expression, with highest expression in amygdala. Also expressed in fetal tissues, mainly in liver and brain.
  • Domain

    Both the N-terminal (up to position 79) and the C-terminal (from position 304) sequences are required for interaction with SSX2.
  • Cellular localization

    Cell junction > adherens junction. Nucleus. Not found at cell-matrix AJs.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADIP antibody
    • ADIP_HUMAN antibody
    • Afadin and alpha actinin binding protein antibody
    • Afadin DIL domain interacting protein antibody
    • Afadin DIL domain-interacting protein antibody
    • Afadin- and alpha-actinin-binding protein antibody
    • FLJ10848 antibody
    • KIAA0923 antibody
    • MGC75026 antibody
    • OTTHUMP00000011574 antibody
    • SSX 2 interacting protein antibody
    • SSX2 interacting protein antibody
    • SSX2-interacting protein antibody
    • SSX2IP antibody
    • Synovial sarcoma X breakpoint 2 interacting protein antibody
    see all

Images

  • All lanes : Anti-SSX2IP antibody [EPR16979] (ab199425) at 1/10000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK293 (Human embryonic kidney) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 68, 71 kDa
    Observed band size: 68, 71 kDa


    Exposure time: 1 minute


    Based on the sequence analysis, ab199425 recognizes two isoforms with the predicted MWs of 71KDa, and 68KDa, respectively.

  • Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling SSX2IP with ab199425 at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • SSX2IP was immunoprecipitated from 1mg of HEK293 (Human embryonic kidney) whole cell lysate with ab199425 at 1/150 dilution. Western blot was performed from the immunoprecipitate using ab199425 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Input HEK293 whole cell lysate (10 µg). Lane 2: HEK293 whole cell lysate folowing precipitation. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199425 in HEK293 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST. 10 second exposure.

  • Anti-SSX2IP antibody [EPR16979] (ab199425) at 1/20000 dilution + Human testis lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 68, 71 kDa
    Observed band size: 68, 71 kDa


    Exposure time: 30 seconds


    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SSX2IP with ab199425 at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human kidney tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab199425 has not yet been referenced specifically in any publications.

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