Key features and details
- Rabbit polyclonal to ST14 - Catalytic domain
- Suitable for: ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ST14 antibody - Catalytic domain
See all ST14 primary antibodies
DescriptionRabbit polyclonal to ST14 - Catalytic domain
SpecificityThis antibody does not recognize matriptase 2 (MTSP2).
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab28266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionDegrades extracellular matrix. Proposed to play a role in breast cancer invasion and metastasis. Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site.
Involvement in diseaseDefects in ST14 are a cause of ichthyosis autosomal recessive with hypotrichosis (ARIH) [MIM:610765]. ARIH is a skin disorder characterized by congenital ichthyosis associated with the presence of less than the normal amount of hair.
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 2 CUB domains.
Contains 4 LDL-receptor class A domains.
Contains 1 peptidase S1 domain.
- Information by UniProt
- Epithin antibody
- HAI antibody
- Matriptase antibody
ICC/IF image of ab28266 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28266, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab28266 has been referenced in 3 publications.
- Sun P et al. Regulation of matriptase and HAI-1 system, a novel therapeutic target in human endometrial cancer cells. Oncotarget 9:12682-12694 (2018). PubMed: 29560101
- Kubyshkin AV et al. THE IMPACT OF ALCOHOL ON PRO-METASTATIC N-GLYCOSYLATION IN PROSTATE CANCER. Krim Z Eksp Klin Med 8:11-20 (2018). PubMed: 31131224
- Sun P et al. Decreasing the ratio of matriptase/HAI-1 by downregulation of matriptase as a potential adjuvant therapy in ovarian cancer. Mol Med Rep 14:1465-74 (2016). WB ; Human . PubMed: 27356668