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  1. Link

    st2-antibody-ab228543.pdf

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Cardiovascular Angiogenesis Cytokines Interleukin
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Anti-ST2 antibody (ab228543)

  • Datasheet
  • SDS
Reviews (9) Submit a question References (3)

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Western blot - Anti-ST2 antibody (ab228543)
  • Western blot - Anti-ST2 antibody (ab228543)

Key features and details

  • Rabbit polyclonal to ST2
  • Suitable for: WB
  • Reacts with: Mouse, Rat
  • Isotype: IgG

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Overview

  • Product name

    Anti-ST2 antibody
    See all ST2 primary antibodies
  • Description

    Rabbit polyclonal to ST2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment within Human ST2 (internal sequence). The exact sequence is proprietary.
    Database link: Q01638

  • Positive control

    • WB: RAW 264.7 whole cell lysate; rat colon tissue extract.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Cytokines
    • Interleukin
    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other
    • Immunology
    • Innate Immunity
    • TLR Signaling

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • RAW 264.7 whole cell lysate (ab7187)
  • Recombinant Protein

    • Recombinant human ST2 protein (Fc Chimera Active) (ab219662)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab228543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/500 - 1/3000. Predicted molecular weight: 63 kDa.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 63 kDa.

Target

  • Function

    Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function.
  • Tissue specificity

    Highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. Isoform A is prevalently expressed in the lung, testis, placenta, stomach and colon. Isoform B is more abundant in the brain, kidney and the liver. Isoform C is not detected in brain, heart, liver, kidney and skeletal muscle.
  • Sequence similarities

    Belongs to the interleukin-1 receptor family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 TIR domain.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession Q01638 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9173 Human
    • Entrez Gene: 17082 Mouse
    • Entrez Gene: 25556 Rat
    • Omim: 601203 Human
    • SwissProt: Q01638 Human
    • SwissProt: P14719 Mouse
    • SwissProt: Q62611 Rat
    • Unigene: 66 Human
    • Unigene: 289824 Mouse
    • Unigene: 10072 Rat
    see all
  • Alternative names

    • DER-4 antibody
    • DER4 antibody
    • FIT 1 antibody
    • Growth stimulation expressed antibody
    • homolog of mouse growth stimulation-expressed antibody
    • Il1rl1 antibody
    • IL33R antibody
    • ILRL1_HUMAN antibody
    • Interleukin 1 receptor like 1 antibody
    • interleukin 1 receptor related protein antibody
    • Interleukin-1 receptor-like 1 antibody
    • Protein ST2 antibody
    • ST2 antibody
    • ST2L antibody
    • ST2V antibody
    • T1 antibody
    • T1 protein antibody
    see all

Images

  • Western blot - Anti-ST2 antibody (ab228543)
    Western blot - Anti-ST2 antibody (ab228543)
    Anti-ST2 antibody (ab228543) at 1/500 dilution + RAW 264.7 (house macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 63 kDa



    10% SDS-PAGE

  • Western blot - Anti-ST2 antibody (ab228543)
    Western blot - Anti-ST2 antibody (ab228543)
    Anti-ST2 antibody (ab228543) at 1/500 dilution + Rat colon tissue extract at 50 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 63 kDa



    10% SDS-PAGE

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (3)

Publishing research using ab228543? Please let us know so that we can cite the reference in this datasheet.

ab228543 has been referenced in 3 publications.

  • Zhang K  et al. Methionine enkephalin (MENK) regulates the immune pathogenesis of type 2 diabetes mellitus via the IL-33/ST2 pathway. Int Immunopharmacol 73:23-40 (2019). PubMed: 31078923
  • Yang J  et al. LncRNA ANRIL knockdown relieves myocardial cell apoptosis in acute myocardial infarction by regulating IL-33/ST2. Cell Cycle 18:3393-3403 (2019). PubMed: 31674275
  • Liang Y  et al. Elevated IL-33 promotes expression of MMP2 and MMP9 via activating STAT3 in alveolar macrophages during LPS-induced acute lung injury. Cell Mol Biol Lett 23:52 (2018). PubMed: 30410547

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-ST2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - other (EMT-6 tumors taken out of Balb/C mice)
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Glycin gel, semi dry transfer 35min 20V, PVDF membrane)
Loading amount
50 µg
Specification
EMT-6 tumors taken out of Balb/C mice
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 15 2019

Western blot abreview for Anti-ST2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116, HT29)
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Glycin gels)
Loading amount
50 µg
Specification
HCT116, HT29
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 12 2018

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