Overview

  • Product name

  • Description

    Rabbit polyclonal to ST2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment within Human ST2 (internal sequence). The exact sequence is proprietary.
    Database link: Q01638

  • Positive control

    • WB: RAW 264.7 whole cell lysate; rat colon tissue extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab228543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 63 kDa.

Target

  • Function

    Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function.
  • Tissue specificity

    Highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. Isoform A is prevalently expressed in the lung, testis, placenta, stomach and colon. Isoform B is more abundant in the brain, kidney and the liver. Isoform C is not detected in brain, heart, liver, kidney and skeletal muscle.
  • Sequence similarities

    Belongs to the interleukin-1 receptor family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 TIR domain.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • DER-4 antibody
    • DER4 antibody
    • FIT 1 antibody
    • Growth stimulation expressed antibody
    • homolog of mouse growth stimulation-expressed antibody
    • Il1rl1 antibody
    • IL33R antibody
    • ILRL1_HUMAN antibody
    • Interleukin 1 receptor like 1 antibody
    • interleukin 1 receptor related protein antibody
    • Interleukin-1 receptor-like 1 antibody
    • Protein ST2 antibody
    • ST2 antibody
    • ST2L antibody
    • ST2V antibody
    • T1 antibody
    • T1 protein antibody
    see all

Images

  • Anti-ST2 antibody (ab228543) at 1/500 dilution + RAW 264.7 (house macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 63 kDa



    10% SDS-PAGE

  • Anti-ST2 antibody (ab228543) at 1/500 dilution + Rat colon tissue extract at 50 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 63 kDa



    10% SDS-PAGE

References

This product has been referenced in:

  • Liang Y  et al. Elevated IL-33 promotes expression of MMP2 and MMP9 via activating STAT3 in alveolar macrophages during LPS-induced acute lung injury. Cell Mol Biol Lett 23:52 (2018). Read more (PubMed: 30410547) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - other (EMT-6 tumors taken out of Balb/C mice)
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Glycin gel, semi dry transfer 35min 20V, PVDF membrane)
Loading amount
50 µg
Specification
EMT-6 tumors taken out of Balb/C mice
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Mar 15 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human breast cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA ph:9
Permeabilization
No
Specification
human breast cancer
Blocking step
Innovex Background Buster as blocking agent for 30 minute(s) · Concentration: 100%
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jan 07 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Rat brain sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
Rat brain sections
Blocking step
Innovex Background Buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 13 2018

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116, HT29)
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Glycin gels)
Loading amount
50 µg
Specification
HCT116, HT29
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Nov 12 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse brain section)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
mouse brain section
Blocking step
innovex background buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 12 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up