Anti-ST2 antibody (ab228543)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-ST2 antibody
See all ST2 primary antibodies -
Description
Rabbit polyclonal to ST2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human
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Immunogen
Recombinant fragment within Human ST2 (internal sequence). The exact sequence is proprietary.
Database link: Q01638 -
Positive control
- WB: RAW 264.7 whole cell lysate; rat colon tissue extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab228543 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 63 kDa. |
Target
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Function
Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function. -
Tissue specificity
Highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. Isoform A is prevalently expressed in the lung, testis, placenta, stomach and colon. Isoform B is more abundant in the brain, kidney and the liver. Isoform C is not detected in brain, heart, liver, kidney and skeletal muscle. -
Sequence similarities
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 9173 Human
- Entrez Gene: 17082 Mouse
- Entrez Gene: 25556 Rat
- Omim: 601203 Human
- SwissProt: Q01638 Human
- SwissProt: P14719 Mouse
- SwissProt: Q62611 Rat
- Unigene: 66 Human
see all -
Alternative names
- DER-4 antibody
- DER4 antibody
- FIT 1 antibody
see all
Images
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Western blot - Anti-ST2 antibody (ab228543)Anti-ST2 antibody (ab228543) at 1/500 dilution + RAW 264.7 (house macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 30 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 63 kDa10% SDS-PAGE
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Western blot - Anti-ST2 antibody (ab228543)Anti-ST2 antibody (ab228543) at 1/500 dilution + Rat colon tissue extract at 50 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 63 kDa10% SDS-PAGE
References
This product has been referenced in:
- Liang Y et al. Elevated IL-33 promotes expression of MMP2 and MMP9 via activating STAT3 in alveolar macrophages during LPS-induced acute lung injury. Cell Mol Biol Lett 23:52 (2018). Read more (PubMed: 30410547) »
