Western blot abreview for Anti-ST2 antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116, HT29)
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Glycin gels)
Loading amount
50 µg
Specification
HCT116, HT29
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
6 minute(s) and 0 second(s)
Bands
Specific: 65, 30 kDa

Additional data

Abcam response
Thank you for your valuable feedback. We have not previously tested this antibody in human samples, and we are pleased to know that it worked fine for this species. We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.

Abcam user community

Verified customer

Submitted Nov 12 2018

Sign up