Anti-ST2 antibody - BSA free (ab25877)
Key features and details
- Rabbit polyclonal to ST2 - BSA free
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ST2 antibody - BSA free
See all ST2 primary antibodies -
Description
Rabbit polyclonal to ST2 - BSA free -
Host species
Rabbit -
Specificity
ab25877 reacts all three known ST2 isoforms. -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Mouse ST2 aa 40-90 (N terminal).
(Peptide available asab39855) -
Positive control
- IHC-P: Human lung cancer, Human liver and Mouse kidney tissues. WB: Hela, HepG2, 3T3/NIH, YB2/0 whole cell lysates. ICC/IF: Mouse kidney cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ST2 Antibody is affinity chromatography purified via peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (2) |
Use a concentration of 2 µg/ml.
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ICC/IF |
Use a concentration of 20 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 40 kDa.
ab25877 at 2ug/ml. 1 hour incubation at room temperature in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. |
Notes |
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IHC-P
Use a concentration of 2 µg/ml. |
ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 40 kDa. ab25877 at 2ug/ml. 1 hour incubation at room temperature in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. |
Target
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Function
Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function. -
Tissue specificity
Highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. Isoform A is prevalently expressed in the lung, testis, placenta, stomach and colon. Isoform B is more abundant in the brain, kidney and the liver. Isoform C is not detected in brain, heart, liver, kidney and skeletal muscle. -
Sequence similarities
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 9173 Human
- Entrez Gene: 17082 Mouse
- Entrez Gene: 25556 Rat
- Omim: 601203 Human
- SwissProt: Q01638 Human
- SwissProt: P14719 Mouse
- SwissProt: Q62611 Rat
- Unigene: 66 Human
see all -
Alternative names
- DER-4 antibody
- DER4 antibody
- FIT 1 antibody
see all
Images
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Human lung cancer tissue stained for ST2 using ab25877 at 5 μg/ml in immunohistochemical analysis.
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Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human lung cancer tissue labeling ST2 with Anti-ST2 antibody (ab25877) at 5μg/ml.
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Immunohistochemistry of ST2 in human liver tissue with ST2 antibody at 5 µg/ml.
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ab25877 at 2µg/ml staining ST2 in mouse kidney tissue by IHC
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All lanes : Anti-ST2 antibody - BSA free (ab25877) at 0.5 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 4 : YB2/0 whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution
Predicted band size: 40 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteWestern blot analysis labeling ST2 with ab25877 at 0.5 μg/mL in 5% NFDM/TBST, followed by secondary antibody, incubated at room temperature for 1hr.
Running condition: 10% gel, 130V for 1.5 hrs.
Transfer condition: Wet, 250mA, 2 hrs (NC membrane).
Blocking condition: 5% non-fat milk in TBS at 4°C overnight.
Washing condition: 5ml TBST, 4 x 5 min.
Exposure system: GE ECL 1 min.
Target bands were observed at 70kD in Human, mouse and rat cells and at 39kD in mouse cells.
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Mouse kidney cells stained for ST2 using ab25877 at 20 μg/ml in ICC/IF.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (25)
ab25877 has been referenced in 25 publications.
- Wu Q et al. A Single Administration of Riluzole Applied Acutely After Spinal Cord Injury Attenuates Pro-inflammatory Activity and Improves Long-Term Functional Recovery in Rats. J Mol Neurosci 72:730-740 (2022). PubMed: 34988900
- Zeng F et al. An Autocrine Circuit of IL-33 in Keratinocytes Is Involved in the Progression of Psoriasis. J Invest Dermatol 141:596-606.e7 (2021). PubMed: 32853659
- Hu B et al. Effect of parasympathetic inhibition on expression of ILC2 cells in a mouse model of allergic rhinitis. World Allergy Organ J 14:100582 (2021). PubMed: 34659628
- Geng D et al. Induction of IRAK-M in melanoma induces caspase-3 dependent apoptosis by reducing TRAF6 and calpastatin levels. Commun Biol 3:306 (2020). PubMed: 32533049
- Engler AE et al. Airway-Associated Macrophages in Homeostasis and Repair. Cell Rep 33:108553 (2020). PubMed: 33378665