Overview

  • Product name

  • Description

    Rabbit polyclonal to ST3GAL2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant protein fragment contain a sequence corresponding to a region within amino acids 68 and 297 of Human ST3GAL2 (UniProt ID: Q16842).

  • Positive control

    • 293T, A431, HeLaS3, HepG2, MOLT4, Raji and H1299 whole cell lysates

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 40 kDa.

Target

  • Function

    It may be responsible for the synthesis of the sequence NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- found in terminal carbohydrate groups of certain glycoproteins, oligosaccharides and glycolipids. SIAT4A and SIAT4B sialylate the same acceptor substrates but exhibit different Km values.
  • Tissue specificity

    Highly expressed in skeletal muscle and heart and to a much lesser extent in brain, placenta, liver and pancreas. Scarcely detectable in lung and kidney.
  • Pathway

    Protein modification; protein glycosylation.
  • Sequence similarities

    Belongs to the glycosyltransferase 29 family.
  • Post-translational
    modifications

    The soluble form derives from the membrane form by proteolytic processing.
  • Cellular localization

    Golgi apparatus > Golgi stack membrane. Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3-GalNAc-alpha-2 antibody
    • 3-sialyltransferase 2 antibody
    • 3-sialyltransferase antibody
    • 3-ST 2 antibody
    • Alpha 2 antibody
    • Alpha 2,3-ST antibody
    • Beta-galactoside alpha-2 antibody
    • Beta-galactoside alpha-2,3-sialyltransferase antibody
    • CMP N-acetylneuraminate beta galactosamide alpha 2,3 sialyltransferase antibody
    • CMP-N-acetylneuraminate-beta-galactosamide-alpha-2 antibody
    • Gal NAc6S antibody
    • Gal-beta-1 antibody
    • Gal-NAc6S antibody
    • SIA4B_HUMAN antibody
    • sialyltransferase 4B (beta-galactosidase alpha-2,3-sialytransferase) antibody
    • Sialyltransferase 4B antibody
    • SIAT4-B antibody
    • SIAT4B antibody
    • ST3 beta-galactoside alpha-2,3-sialyltransferase 2 antibody
    • ST3Gal II antibody
    • ST3GAL2 antibody
    • ST3GalA.2 antibody
    • ST3GalII antibody
    see all

Images

  • Anti-ST3GAL2 antibody (ab96028) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 40 kDa



    10% SDS-PAGE

References

This product has been referenced in:

  • Li Y  et al. Alpha-2, 3-sialyltransferases regulate the multidrug resistance of chronic myeloid leukemia through miR-4701-5p targeting ST3GAL1. Lab Invest 96:731-40 (2016). Read more (PubMed: 27088512) »
  • Ali MF  et al. Golgi Phosphoprotein 3 Determines Cell Binding Properties under Dynamic Flow by Controlling Golgi Localization of Core 2 N-Acetylglucosaminyltransferase 1. J Biol Chem 287:39564-77 (2012). ICC/IF . Read more (PubMed: 23027862) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Human melanoma cell line)
Gel Running Conditions
Reduced Denaturing (5-20%)
Loading amount
20 µg
Specification
Human melanoma cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 14 2019

Application
IHC - Wholemount
Sample
Human Tissue (Melanoma human cells FFPE blocks)
Specification
Melanoma human cells FFPE blocks

Abcam user community

Verified customer

Submitted May 14 2019

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