Key features and details
- Rabbit polyclonal to ST3GAL3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ST3GAL3 antibody
See all ST3GAL3 primary antibodies
DescriptionRabbit polyclonal to ST3GAL3
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to a region within amino acids 141-375 of Human ST3GAL3 (UniProt: Q11203).
- NT2D1 and IMR32 whole cell lysates; MCF7 cells; Human Adrenal gland tumor tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 42 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
FunctionCatalyzes the formation of the NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc-, NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- or NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. The highest activity is toward Gal-beta-1,3-GlcNAc and the lowest toward Gal-beta-1,3-GalNAc.
Tissue specificityHighly expressed in adult skeletal muscle and in all fetal tissues examined and to a much lesser extent in placenta, lung and liver.
PathwayProtein modification; protein glycosylation.
Involvement in diseaseMental retardation, autosomal recessive 12
Epileptic encephalopathy, early infantile, 15
Sequence similaritiesBelongs to the glycosyltransferase 29 family.
modificationsThe soluble form derives from the membrane form by proteolytic processing.
Cellular localizationGolgi apparatus, Golgi stack membrane. Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
- Information by UniProt
- 3 sialyltransferase antibody
- 3(4) GlcNAc alpha-2 antibody
- 3-sialyltransferase 3 antibody
Immunohistochemical analysis of paraffin-embedded Human adrenal gland tumor labeling ST3GAL3 with ab155022 at 1/250.
All lanes : Anti-ST3GAL3 antibody (ab155022) at 1/5000 dilution
Lane 1 : NT2D1 whole cell lysate
Lane 2 : IMR32 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 42 kDa
10% SDS PAGE
Immunofluorescence analysis of methanol-fixed MCF7 labeling ST3GAL3 with ab155022 at 1/500 dilution. Lower image shows costaining with Hoechst 33342.
ab155022 has not yet been referenced specifically in any publications.