Overview

  • Product name
  • Description
    Rabbit polyclonal to ST3GAL3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 141-375 of Human ST3GAL3 (UniProt: Q11203).

  • Positive control
    • NT2D1 and IMR32 whole cell lysates; MCF7 cells; Human Adrenal gland tumor tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 42 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Catalyzes the formation of the NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc-, NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- or NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. The highest activity is toward Gal-beta-1,3-GlcNAc and the lowest toward Gal-beta-1,3-GalNAc.
  • Tissue specificity
    Highly expressed in adult skeletal muscle and in all fetal tissues examined and to a much lesser extent in placenta, lung and liver.
  • Pathway
    Protein modification; protein glycosylation.
  • Involvement in disease
    Mental retardation, autosomal recessive 12
    Epileptic encephalopathy, early infantile, 15
  • Sequence similarities
    Belongs to the glycosyltransferase 29 family.
  • Post-translational
    modifications
    The soluble form derives from the membrane form by proteolytic processing.
  • Cellular localization
    Golgi apparatus, Golgi stack membrane. Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 sialyltransferase antibody
    • 3(4) GlcNAc alpha-2 antibody
    • 3-sialyltransferase 3 antibody
    • 3-sialyltransferase antibody
    • 3-ST 3 antibody
    • 4-galactoside alpha-2 antibody
    • 4GlcNAc alpha 2 3 sialyltransferase antibody
    • Alpha 2 3 sialyltransferase II antibody
    • Alpha 2 3 sialyltransferase III antibody
    • Alpha 2 3 ST 3 antibody
    • Alpha 2 antibody
    • Beta galactoside alpha 3 sialyltransferase 3 antibody
    • Beta-galactoside alpha-2 antibody
    • CMP N acetylneuraminate beta 1 4 galactoside alpha 2 3 sialyltransferase antibody
    • CMP-N-acetylneuraminate-beta-1 antibody
    • EC 2.4.99.6 antibody
    • Gal beta 1 3 antibody
    • Gal beta 1 3(4) GlcNAc alpha 2 3 sialyltransferase antibody
    • Gal beta 1 3(4)GlcNAc alpha 2 3 sialyltransferase antibody
    • Gal beta-1 antibody
    • N acetyllactosaminide alpha 2 3 sialyltransferase antibody
    • N-acetyllactosaminide alpha-2 antibody
    • OTTHUMP00000008820 antibody
    • OTTHUMP00000008821 antibody
    • OTTHUMP00000008822 antibody
    • OTTHUMP00000008823 antibody
    • Sialyltransferase 6 (N acetyllacosaminide alpha 2 3 sialyltransferase) antibody
    • Sialyltransferase 6 antibody
    • SIAT6 antibody
    • SIAT6_HUMAN antibody
    • ST3 beta galactoside alpha 2 3 sialyltransferase 3 antibody
    • ST3 beta galactoside alpha 2,3 sialyltransferase 3 antibody
    • ST3Gal III antibody
    • St3gal3 antibody
    • ST3GALII antibody
    • ST3GalIII antibody
    • ST3N antibody
    see all

Images

  • All lanes : Anti-ST3GAL3 antibody (ab155022) at 1/5000 dilution

    Lane 1 : NT2D1 whole cell lysate
    Lane 2 : IMR32 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 42 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human adrenal gland tumor labeling ST3GAL3 with ab155022 at 1/250.
  • Immunofluorescence analysis of methanol-fixed MCF7 labeling ST3GAL3 with ab155022 at 1/500 dilution. Lower image shows costaining with Hoechst 33342.

References

ab155022 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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