Anti-ST6GalNAc I antibody (ab229816)
Key features and details
- Rabbit polyclonal to ST6GalNAc I
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ST6GalNAc I antibody
See all ST6GalNAc I primary antibodies -
Description
Rabbit polyclonal to ST6GalNAc I -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human ST6GalNAc I aa 1-250.
Database link: Q9NSC7 -
Positive control
- WB: HepG2 whole cell lysate. IHC-P: Human melanoma cancer and skin tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229816 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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WB |
1/500 - 1/5000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
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Notes |
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IHC-P
1/20 - 1/200. |
WB
1/500 - 1/5000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). |
Target
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Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the glycosyltransferase 29 family. -
Cellular localization
Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 55808 Human
- Omim: 610138 Human
- SwissProt: Q9NSC7 Human
- Unigene: 105352 Human
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Alternative names
- 6-sialyltransferase 1 antibody
- 6-sialyltransferase I antibody
- Alpha N acetylgalactosaminide alpha 2 6 sialyltransferase 1 antibody
see all
Images
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Paraffin-embedded human melanoma cancer tissue stained for ST6GalNAc I using ab229816 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human skin tissue stained for ST6GalNAc I using ab229816 at 1/100 dilution in immunohistochemical analysis.
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Anti-ST6GalNAc I antibody (ab229816) at 1/500 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Secondary
Goat anti-rabbit IgG at 1/50000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab229816 has not yet been referenced specifically in any publications.