Anti-ST7 antibody (ab122459)
Key features and details
- Rabbit polyclonal to ST7
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ST7 antibody -
Description
Rabbit polyclonal to ST7 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: PQARISAAHE ALEINEIRSR VEVPLIASST IWEIKLLPKC ATAY, corresponding to amino acids of Human ST7.
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Positive control
- RT 4 and U 251 cell line lysates; Human heart muscle tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May act as a tumor suppressor. -
Tissue specificity
Ubiquitously expressed, with highest levels in heart, liver and pancreas. -
Sequence similarities
Belongs to the ST7 family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 7982 Human
- Omim: 600833 Human
- SwissProt: Q9NRC1 Human
- Unigene: 368131 Human
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Alternative names
- ETS7q antibody
- FAM4A antibody
- FAM4A1 antibody
see all
Images
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All lanes : Anti-ST7 antibody (ab122459) at 1/250 dilution
Lane 1 : RT 4 cell line lysate
Lane 2 : U 251 MG cell line lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate -
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122459 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab122459, at 1/500, staining ST7 in paraffin embedded Human heart muscle tissue by Immunohistochemistry.
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Immunohistochemical staining of Human pancreas shows moderate membranous positivity using ab122459
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab122459 has not yet been referenced specifically in any publications.