Overview

  • Product name
  • Description
    Rabbit polyclonal to STAC
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 17-266 of Human STAC (Q99469).

  • Positive control
    • HepG2 whole cell lysate. Mouse brain whole cell lysate. HepG2 cells. MCF xenograft tissue.
  • General notes
    Keep as concentrated solution.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 45 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Probably involved in a neuron-specific signal transduction.
  • Sequence similarities
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 SH3 domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ32331 antibody
    • SH3 and cysteine rich domain antibody
    • SH3 and cysteine rich domain containing protein antibody
    • SH3 and cysteine-rich domain-containing protein antibody
    • SRC homology 3 and cysteine rich domain protein antibody
    • Src homology 3 and cysteine-rich domain-containing protein antibody
    • Src homology three (SH3) and cysteine rich domain antibody
    • STAC 1 antibody
    • STAC antibody
    • STAC_HUMAN antibody
    • STAC1 antibody
    see all

Images

  • Immunofluorescence analysis of methanol fixed HepG2 cells, labelling STAC with ab155006 at a 1/500 dilution. Lower panel costained with Hoechst 33342.
  • Anti-STAC antibody (ab155006) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 45 kDa



    10% SDS PAGE
  • Anti-STAC antibody (ab155006) at 1/1000 dilution + mouse brain whole cell lysate at 50 µg

    Predicted band size: 45 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded MCF xenograft tissue, labelling STAC with ab155006 at a 1/100 dilution.

References

ab155006 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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