Recombinant Anti-STAC antibody [EPR12805] - N-terminal (ab181157)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12805] to STAC - N-terminal
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-STAC antibody [EPR12805] - N-terminal
See all STAC primary antibodies -
Description
Rabbit monoclonal [EPR12805] to STAC - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- U87-MG, 293, Human fetal brain or A431 lysate, A431 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12805 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
|
ICC/IF |
1/100 - 1/250.
|
Notes |
---|
Flow Cyt (Intra)
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
ICC/IF
1/100 - 1/250. |
Target
-
Function
Probably involved in a neuron-specific signal transduction. -
Sequence similarities
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 SH3 domain. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 6769 Human
- Entrez Gene: 20840 Mouse
- Entrez Gene: 363152 Rat
- Omim: 602317 Human
- SwissProt: Q99469 Human
- SwissProt: P97306 Mouse
- Unigene: 56045 Human
- Unigene: 1414 Mouse
-
Alternative names
- FLJ32331 antibody
- SH3 and cysteine rich domain antibody
- SH3 and cysteine rich domain containing protein antibody
see all
Images
-
Immunofluorescence analysis of A431 cells (fixative 4% paraformaldehyde) labeling STAC with ab181157 at a 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution. Counter stained with Dapi (blue).
-
Intracellular Flow Cytometry analysis of A431 cells labeling STAC using ab181157at a 1/20 dilution (red) or aRabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary usedat a 1/150 dilution.
-
All lanes : Anti-STAC antibody [EPR12805] - N-terminal (ab181157) at 1/2000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDa -
All lanes : Anti-STAC antibody [EPR12805] - N-terminal (ab181157) at 1/10000 dilution
Lane 1 : U87-MG lysate
Lane 2 : 293 lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDa
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (1)
ab181157 has been referenced in 1 publication.
- Niu J et al. Allosteric regulators selectively prevent Ca2+-feedback of CaV and NaV channels. Elife 7:N/A (2018). PubMed: 30198845