Product nameStains-All, cationic carbocyanine dye
DescriptionCationic carbocyanine dye for differential staining of nuclei acids and proteins.
- 1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]naphtho[1,2-d]thiazolium bromide
Cationic carbocyanine dye used for differential staining of nuclei acids and proteins with RNA staining blue/purple, DNA staining blue, and proteins staining red. Ideal dye for the visualization and identification of proteins on polyacrylamide gels because it differentiates between highly acidic proteins which stain blue and less acidic proteins which stain pink. Staining of Ca2+-binding proteins in muscle extracts was 50 to 100% more intense than obtained with Coomassie blue staining. When employed on polyacrylamide gels, the intensity of Stains-All can be increased by the addition of silver nitrate.
Chemical name1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazol-2(1H)-ylidene)-2-methyl-1-propenyl]naphtho[1,2-d]thiazolium bromide
Storage instructionsStore at Room Temperature. The product can be stored for up to 12 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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This product has been referenced in:
- Goldberg HA & Warner KJ The staining of acidic proteins on polyacrylamide gels: enhanced sensitivity and stability of "Stains-all" staining in combination with silver nitrate. Anal Biochem 251:227-33 (1997). Read more (PubMed: 9299020) »
- Sharma Y et al. Studies on the interaction of the dye, stains-all, with individual calcium-binding domains of calmodulin. FEBS Lett 326:59-64 (1993). Read more (PubMed: 8325390) »
- Campbell KP et al. Staining of the Ca2+-binding proteins, calsequestrin, calmodulin, troponin C, and S-100, with the cationic carbocyanine dye "Stains-all". J Biol Chem 258:11267-73 (1983). Read more (PubMed: 6193121) »