Key features and details
- Cationic carbocyanine dye for differential staining of nuclei acids and proteins.
- CAS Number: 7423-31-6
- Purity: > 95%
- Form / State: Solid
- Source: Synthetic
Product nameStains-All, cationic carbocyanine dye
DescriptionCationic carbocyanine dye for differential staining of nuclei acids and proteins.
- 1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]naphtho[1,2-d]thiazolium bromide
Cationic carbocyanine dye used for differential staining of nuclei acids and proteins with RNA staining blue/purple, DNA staining blue, and proteins staining red. Ideal dye for the visualization and identification of proteins on polyacrylamide gels because it differentiates between highly acidic proteins which stain blue and less acidic proteins which stain pink. Staining of Ca2+-binding proteins in muscle extracts was 50 to 100% more intense than obtained with Coomassie blue staining. When employed on polyacrylamide gels, the intensity of Stains-All can be increased by the addition of silver nitrate.
Chemical name1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazol-2(1H)-ylidene)-2-methyl-1-propenyl]naphtho[1,2-d]thiazolium bromide
Storage instructionsStore at Room Temperature. The product can be stored for up to 12 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab146278 has not yet been referenced specifically in any publications.