Product nameAnti-STAM1 antibody
See all STAM1 primary antibodies
DescriptionRabbit polyclonal to STAM1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide, corresponding to a region within amino acids 300-350 of Human STAM1 according to NP_003464.1.
- Whole cell lysate from HeLa, 293T and Jurkat cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab117813 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 58 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionInvolved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the STAM family.
Contains 1 ITAM domain.
Contains 1 SH3 domain.
Contains 1 UIM (ubiquitin-interacting motif) repeat.
Contains 1 VHS domain.
modificationsPhosphorylated on Tyr-198. Phosphorylated in response to IL2, IL3, IL4, IL7, CSF2/GM-CSF, EGF and PDGFB. Phosphorylated by activated PDGFRB.
Cellular localizationCytoplasm. Early endosome membrane.
- Information by UniProt
- HSE1 homolog antibody
- Signal transducing adapter molecule 1 antibody
- signal transducing adaptor molecule (SH3 domain and ITAM motif) 1 antibody
All lanes : Anti-STAM1 antibody (ab117813) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from 293T cells at 50 µg
Lane 4 : Whole cell lysate from Jurkat cells at 50 µg
Developed using the ECL technique.
Predicted band size: 58 kDa
Exposure time: 30 seconds
ab117813 at 1 µg/ml staining STAM1 by WB, following immunoprecipitation of whole cell lysate from HeLa cells.
Lane 1; IP using ab117813 at 6 µg/mg lysate.
Lane 1; IP using an antibody to a downstream epitope.
Lane 2; IP using an antibody to an alternative downstream epitope.
Lane 3; IP using control IgG.
1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised ECL with a 10 second exposure.
ab117813 has not yet been referenced specifically in any publications.