Anti-STAM1 antibody (ab155527)
Key features and details
- Rabbit polyclonal to STAM1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-STAM1 antibody
See all STAM1 primary antibodies -
Description
Rabbit polyclonal to STAM1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment corresponding to a region within amino acids 147-362 of Human STAM (UniProt Q92783).
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Positive control
- Jurkat, Raji, K562 and NCI-H929 whole cell lysates; Human colon carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 59 kDa.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 59 kDa. |
IHC-P
1/100 - 1/1000. |
Target
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Function
Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the STAM family.
Contains 1 ITAM domain.
Contains 1 SH3 domain.
Contains 1 UIM (ubiquitin-interacting motif) repeat.
Contains 1 VHS domain. -
Post-translational
modificationsPhosphorylated on Tyr-198. Phosphorylated in response to IL2, IL3, IL4, IL7, CSF2/GM-CSF, EGF and PDGFB. Phosphorylated by activated PDGFRB. -
Cellular localization
Cytoplasm. Early endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 8027 Human
- Entrez Gene: 20844 Mouse
- Omim: 601899 Human
- SwissProt: Q92783 Human
- SwissProt: P70297 Mouse
- Unigene: 335391 Human
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Alternative names
- DKFZp686J2352 antibody
- HSE1 homolog antibody
- OTTHUMP00000019237 antibody
see all
Images
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All lanes : Anti-STAM1 antibody (ab155527) at 1/1000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : NCI-H929 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 59 kDa
10% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling STAM with ab155527 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155527 has not yet been referenced specifically in any publications.