Key features and details
- Rabbit polyclonal to STAM1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-STAM1 antibody
See all STAM1 primary antibodies
DescriptionRabbit polyclonal to STAM1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment corresponding to a region within amino acids 147-362 of Human STAM (UniProt Q92783).
- Jurkat, Raji, K562 and NCI-H929 whole cell lysates; Human colon carcinoma tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 59 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionInvolved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the STAM family.
Contains 1 ITAM domain.
Contains 1 SH3 domain.
Contains 1 UIM (ubiquitin-interacting motif) repeat.
Contains 1 VHS domain.
modificationsPhosphorylated on Tyr-198. Phosphorylated in response to IL2, IL3, IL4, IL7, CSF2/GM-CSF, EGF and PDGFB. Phosphorylated by activated PDGFRB.
Cellular localizationCytoplasm. Early endosome membrane.
- Information by UniProt
- DKFZp686J2352 antibody
- HSE1 homolog antibody
- OTTHUMP00000019237 antibody
All lanes : Anti-STAM1 antibody (ab155527) at 1/1000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : NCI-H929 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 59 kDa
10% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling STAM with ab155527 at 1/500 dilution.
ab155527 has not yet been referenced specifically in any publications.