Overview

  • Product name

  • Description

    Rabbit polyclonal to STAM2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Sheep, Rabbit, Horse, Cow, Cat, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human STAM2 aa 175-225. The exact sequence is proprietary.
    Sequence:

    ELSLQE QKQQHTETKS LYPSSEIQLN NKVARKVRAL YDFEAVEDNE LTFKH


    Database link: O75886

  • Positive control

    • HeLa, Jurkat and 293T whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH: 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab206696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the STAM family.
    Contains 1 ITAM domain.
    Contains 1 SH3 domain.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
    Contains 1 VHS domain.
  • Domain

    The VHS and UIM domains mediate the interaction with ubiquitinated proteins.
    The SH3 domain mediates the interaction with USP8.
    Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
  • Post-translational
    modifications

    Phosphorylated in response to IL-2, GM-CSF, EGF and PDGF.
  • Cellular localization

    Cytoplasm. Early endosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp564C047 antibody
    • Hbp antibody
    • Hrs-binding protein antibody
    • HSE1 homolog antibody
    • Signal transducing adapter molecule 2 antibody
    • Signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 antibody
    • Signal transducing adaptor molecule 2A antibody
    • Signal transducing adaptor molecule 2B antibody
    • Signal transducing adaptor molecule antibody
    • STAM like protein containing SH3 and ITAM domains 2 antibody
    • STAM-2 antibody
    • Stam2 antibody
    • STAM2_HUMAN antibody
    • STAM2A antibody
    • STAM2B antibody
    see all

Images

  • All lanes : Anti-STAM2 antibody (ab206696) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate in NETN lysis buffer
    Lane 2 : 293T whole cell lysate in NETN lysis buffer
    Lane 3 : Jurkat whole cell lysate in NETN lysis buffer

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Exposure time: 30 seconds
  • Immunoprecipitation analysis of Jurkat cell lysate (in NETN buffer, 0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling STAM2 using ab206696 at 6 μg per reaction (lane 1). A Control IgG was used in lane 2. For blotting immunoprecipitated STAM2, ab206696 was used at 0.4 μg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.

References

ab206696 has not yet been referenced specifically in any publications.

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