Key features and details
- Rabbit polyclonal to STAM2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Product nameAnti-STAM2 antibody
See all STAM2 primary antibodies
DescriptionRabbit polyclonal to STAM2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human, Pig
Recombinant fragment (His-T7-tag) corresponding to Human STAM2 aa 1-377. (Expressed in E.coli).
MPLFTANPFEQDVEKATNEYNTTEDWSLIMDICDKVGSTPNGAKDCLKAI MKRVNHKVPHVALQALTLLGACVANCGKIFHLEVCSRDFATEVRAVIKNK AHPKVCEKLKSLMVEWSEEFQKDPQFSLISATIKSMKEEGITFPPAGSQT VSAAAKNGTSSNKNKEDEDIAKAIELSLQEQKQQHTETKSLYPSSEIQLN NKVARKVRALYDFEAVEDNELTFKHGEIIIVLDDSDANWWKGENHRGIGL FPSNFVTTNLNIETEAAAVDKLNVIDDDVEEIKKSEPEPVYIDEDKMDRA LQVLQSIDPTDSKPDSQDLLDLEDICQQMGPMIDEKLEEIDRKHSELSEL NVKVLEALELYNKLVNEAPVYSVYSKL
Database link: O75886
- IHC-P: Human liver and kidney tissues. WB: Recombinant human STAM2 protein; Human serum; Pig liver, brain and kidney lysates; Mouse liver lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab233290 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 58 kDa.|
FunctionInvolved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the STAM family.
Contains 1 ITAM domain.
Contains 1 SH3 domain.
Contains 1 UIM (ubiquitin-interacting motif) repeat.
Contains 1 VHS domain.
DomainThe VHS and UIM domains mediate the interaction with ubiquitinated proteins.
The SH3 domain mediates the interaction with USP8.
Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
modificationsPhosphorylated in response to IL-2, GM-CSF, EGF and PDGF.
Cellular localizationCytoplasm. Early endosome membrane.
- Information by UniProt
- DKFZp564C047 antibody
- Hbp antibody
- Hrs-binding protein antibody
All lanes : Anti-STAM2 antibody (ab233290) at 1 µg/ml
Lane 1 : Human serum
Lane 2 : Pig liver lysate
Lane 3 : Pig brain lysate
Lane 4 : Pig kidney lysate
Lane 5 : Mouse liver lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 58 kDa
Anti-STAM2 antibody (ab233290) at 1 µg/ml + Recombinant human STAM2 protein.
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 58 kDa
Paraffin-embedded human kidney tissue stained for STAM2 using ab233290 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human liver tissue stained for STAM2 using ab233290 at 20 µg/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233290 has not yet been referenced specifically in any publications.