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    stam2-antibody-ab233290.pdf

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Signal Transduction Adapters Cytoplasmic
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Anti-STAM2 antibody (ab233290)

  • Datasheet
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Abpromise

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Western blot - Anti-STAM2 antibody (ab233290)
  • Western blot - Anti-STAM2 antibody (ab233290)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAM2 antibody (ab233290)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAM2 antibody (ab233290)

Key features and details

  • Rabbit polyclonal to STAM2
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human, Pig
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human STAM2 protein (ab132279)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-STAM2 antibody
    See all STAM2 primary antibodies
  • Description

    Rabbit polyclonal to STAM2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human, Pig
  • Immunogen

    Recombinant fragment (His-T7-tag) corresponding to Human STAM2 aa 1-377. (Expressed in E.coli).
    Sequence:

    MPLFTANPFEQDVEKATNEYNTTEDWSLIMDICDKVGSTPNGAKDCLKAI MKRVNHKVPHVALQALTLLGACVANCGKIFHLEVCSRDFATEVRAVIKNK AHPKVCEKLKSLMVEWSEEFQKDPQFSLISATIKSMKEEGITFPPAGSQT VSAAAKNGTSSNKNKEDEDIAKAIELSLQEQKQQHTETKSLYPSSEIQLN NKVARKVRALYDFEAVEDNELTFKHGEIIIVLDDSDANWWKGENHRGIGL FPSNFVTTNLNIETEAAAVDKLNVIDDDVEEIKKSEPEPVYIDEDKMDRA LQVLQSIDPTDSKPDSQDLLDLEDICQQMGPMIDEKLEEIDRKHSELSEL NVKVLEALELYNKLVNEAPVYSVYSKL


    Database link: O75886
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human liver and kidney tissues. WB: Recombinant human STAM2 protein; Human serum; Pig liver, brain and kidney lysates; Mouse liver lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab233290 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Adapters
    • Cytoplasmic

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse liver tissue lysate - total protein (ab29301)
  • Recombinant Protein

    • Recombinant Human STAM2 protein (ab132279)
  • Related Products

    • Recombinant Human STAM2 protein (ab132279)

Applications

Our Abpromise guarantee covers the use of ab233290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 20 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 58 kDa.

Target

  • Function

    Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the STAM family.
    Contains 1 ITAM domain.
    Contains 1 SH3 domain.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
    Contains 1 VHS domain.
  • Domain

    The VHS and UIM domains mediate the interaction with ubiquitinated proteins.
    The SH3 domain mediates the interaction with USP8.
    Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
  • Post-translational
    modifications

    Phosphorylated in response to IL-2, GM-CSF, EGF and PDGF.
  • Cellular localization

    Cytoplasm. Early endosome membrane.
  • Target information above from: UniProt accession O75886 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10254 Human
    • Entrez Gene: 56324 Mouse
    • Omim: 606244 Human
    • SwissProt: O75886 Human
    • SwissProt: O88811 Mouse
    • Unigene: 17200 Human
    • Unigene: 263639 Mouse
    • Alternative names

      • DKFZp564C047 antibody
      • Hbp antibody
      • Hrs-binding protein antibody
      • HSE1 homolog antibody
      • Signal transducing adapter molecule 2 antibody
      • Signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 antibody
      • Signal transducing adaptor molecule 2A antibody
      • Signal transducing adaptor molecule 2B antibody
      • Signal transducing adaptor molecule antibody
      • STAM like protein containing SH3 and ITAM domains 2 antibody
      • STAM-2 antibody
      • Stam2 antibody
      • STAM2_HUMAN antibody
      • STAM2A antibody
      • STAM2B antibody
      see all

    Images

    • Western blot - Anti-STAM2 antibody (ab233290)
      Western blot - Anti-STAM2 antibody (ab233290)
      All lanes : Anti-STAM2 antibody (ab233290) at 1 µg/ml

      Lane 1 : Human serum
      Lane 2 : Pig liver lysate
      Lane 3 : Pig brain lysate
      Lane 4 : Pig kidney lysate
      Lane 5 : Mouse liver lysate

      Secondary
      All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

      Predicted band size: 58 kDa

    • Western blot - Anti-STAM2 antibody (ab233290)
      Western blot - Anti-STAM2 antibody (ab233290)
      Anti-STAM2 antibody (ab233290) at 1 µg/ml + Recombinant human STAM2 protein.

      Secondary
      HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

      Predicted band size: 58 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAM2 antibody (ab233290)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAM2 antibody (ab233290)

      Paraffin-embedded human kidney tissue stained for STAM2 using ab233290 at 20 µg/ml in immunohistochemical analysis. DAB staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAM2 antibody (ab233290)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAM2 antibody (ab233290)

      Paraffin-embedded human liver tissue stained for STAM2 using ab233290 at 20 µg/ml in immunohistochemical analysis. DAB staining.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab233290? Please let us know so that we can cite the reference in this datasheet.

    ab233290 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab233290.
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