Anti-Stanniocalcin 1/STC antibody (ab229477)
Key features and details
- Rabbit polyclonal to Stanniocalcin 1/STC
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-Stanniocalcin 1/STC antibody
See all Stanniocalcin 1/STC primary antibodies -
Description
Rabbit polyclonal to Stanniocalcin 1/STC -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Rhesus monkey
-
Immunogen
Recombinant fragment within Human Stanniocalcin 1/STC (internal sequence). The exact sequence is proprietary.
Database link: P52823 -
Positive control
- IHC-P: Mouse pancreas tissue. WB: Stanniocalcin 1/STC transfected HEK-293T whole cell extract.
-
General notes
Previously labelled as Stanniocalcin 1.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab229477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 28 kDa. |
Target
-
Function
Stimulates renal phosphate reabsorption, and could therefore prevent hypercalcemia. -
Tissue specificity
Expressed in most tissues, with the highest levels in ovary, prostate, heart, kidney and thyroid. In the kidney, expression is confined to the nephron, specifically in the distal convoluted tubule and in the collecting tubule. Not detected in the brain, liver, spleen, peripheral blood leukocytes and adrenal medulla. -
Sequence similarities
Belongs to the stanniocalcin family. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 338078 Cow
- Entrez Gene: 6781 Human
- Entrez Gene: 20855 Mouse
- Entrez Gene: 81801 Rat
- Omim: 601185 Human
- SwissProt: Q9N0T1 Cow
- SwissProt: P52823 Human
- SwissProt: O55183 Mouse
see all -
Alternative names
- Stanniocalcin 1 antibody
- Stanniocalcin antibody
- Stanniocalcin-1 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stanniocalcin 1/STC antibody (ab229477)
Paraffin-embedded mouse pancreas tissue stained for Stanniocalcin 1/STC with ab229477 at a 1/500 dilution in immunohistochemical analysis.
-
Western blot - Anti-Stanniocalcin 1/STC antibody (ab229477)All lanes : Anti-Stanniocalcin 1/STC antibody (ab229477) at 1/5000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : Stanniocalcin 1/STC transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 28 kDa12% SDS-PAGE
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (1)
ab229477 has been referenced in 1 publication.
- Xiong Y & Wang Q STC1 regulates glioblastoma migration and invasion via the TGF-ß/SMAD4 signaling pathway. Mol Med Rep 20:3055-3064 (2019). PubMed: 31432189
