Overview

  • Product name

    Anti-Stanniocalcin 1/STC antibody
    See all Stanniocalcin 1/STC primary antibodies
  • Description

    Rabbit polyclonal to Stanniocalcin 1/STC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Stanniocalcin 1/STC aa 2-51 (N terminal).
    Sequence:

    LQNSAVLLVLVISASATHEAEQNDSVSPRKSRVAAQNSAEVVRCLNSALQ

  • Positive control

    • 721_B cell lysate.
  • General notes

    Previously labelled as Stanniocalcin 1. 

Properties

Applications

Our Abpromise guarantee covers the use of ab83065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-Stanniocalcin 1/STC antibody (ab83065) at 1 µg/ml (5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 28 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?



    Gel concentration: 12%

References

This product has been referenced in:

  • Li Q  et al. Megalin mediates plasma membrane to mitochondria cross-talk and regulates mitochondrial metabolism. Cell Mol Life Sci 75:4021-4040 (2018). Read more (PubMed: 29916093) »
  • Xiang J  et al. Regulation of Intestinal Epithelial Calcium Transport Proteins by Stanniocalcin-1 in Caco2 Cells. Int J Mol Sci 17:N/A (2016). Read more (PubMed: 27409607) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (293T)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Treatment
transiently transfected
Specification
293T
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Eric Routh

Verified customer

Submitted Mar 26 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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