Key features and details
- Rabbit polyclonal to Stanniocalcin 2/STC-2
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Stanniocalcin 2/STC-2 antibody
See all Stanniocalcin 2/STC-2 primary antibodies
DescriptionRabbit polyclonal to Stanniocalcin 2/STC-2
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Stanniocalcin 2/STC-2 aa 200-300 (internal sequence). The exact sequence is proprietary.
Database link: O76061
- Human small intestine tissue. IF/ICC: HeLa cell line.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact firstname.lastname@example.org.
Previously labelled as Stanniocalcin 2.
Storage instructionsStore at +4°C. Do not freeze.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab93723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/50 for 30 mins at RT. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Boil tissue section in 1mM EDTA, pH 8.0 for 10 min followed by cooling at RT for 20 min.
FunctionHas an anti-hypocalcemic action on calcium and phosphate homeostasis.
Tissue specificityExpressed in a variety of tissues including muscle, heart, pancreas, kidney, spleen, prostate, small intestine, colon and peripheral blood leukocytes.
Sequence similaritiesBelongs to the stanniocalcin family.
- Information by UniProt
- Stanniocalcin 2 antibody
- Stanniocalcin related protein antibody
- Stanniocalcin-2 antibody
ab93723 stained HeLa cells. The cells were 4% formaldhyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93723 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
ab93723 at 1/50 dilution staining Stanniocalcin 2/STC-2 in Human small intestine by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab93723 has not yet been referenced specifically in any publications.