Overview

  • Product name

    Anti-Stanniocalcin 2/STC-2 antibody
    See all Stanniocalcin 2/STC-2 primary antibodies
  • Description

    Rabbit polyclonal to Stanniocalcin 2/STC-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Stanniocalcin 2/STC-2 aa 200-300 (internal sequence). The exact sequence is proprietary.
    Database link: O76061

  • Epitope

    Internal region.
  • Positive control

    • Human small intestine tissue. IF/ICC: HeLa cell line.
  • General notes

    Previously labelled as Stanniocalcin 2. 

Properties

Applications

Our Abpromise guarantee covers the use of ab93723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

1/50 for 30 mins at RT. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Boil tissue section in 1mM EDTA, pH 8.0 for 10 min followed by cooling at RT for 20 min.

Target

  • Function

    Has an anti-hypocalcemic action on calcium and phosphate homeostasis.
  • Tissue specificity

    Expressed in a variety of tissues including muscle, heart, pancreas, kidney, spleen, prostate, small intestine, colon and peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the stanniocalcin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Stanniocalcin 2 antibody
    • Stanniocalcin related protein antibody
    • Stanniocalcin-2 antibody
    • Stanniocalcin-related protein antibody
    • Stanniocalcin2 antibody
    • STC 2 antibody
    • STC related protein antibody
    • STC-2 antibody
    • STC-related protein antibody
    • STC2 antibody
    • STC2_HUMAN antibody
    • STCRP antibody
    see all

Images

  • ab93723 stained HeLa cells. The cells were 4% formaldhyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93723 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • ab93723 at 1/50 dilution staining Stanniocalcin 2/STC-2 in Human small intestine by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.

References

ab93723 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry regarding ab93723, Anti-Stannniocalcin 2 antibody. We do not have any experimental data regarding the reactivity of ab93723 with mouse samples. We compared the immunogen with the mouse Stanniocalcin 2 protein (SwissProt O88452) and there is 57% homology so theoretically ab93723 should not react with mouse samples. I hope this information will be helpful. If there is anything else that I can help you with, please do not hesitate to contact me.

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