Overview

  • Product name
    Anti-Staphylococcus aureus antibody
    See all Staphylococcus aureus primary antibodies
  • Description
    Rabbit polyclonal to Staphylococcus aureus
  • Host species
    Rabbit
  • Specificity
    Soluble and structural antigens of the whole bacterium. Antiserum is not absorbed for related microorganisms.
  • Tested applications
    Suitable for: ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Staphylococcus aureus
  • Immunogen

    Staphylococcus aureus strain ATCC 27660 (mixture of intact organisms and lysed cells).

Properties

Applications

Our Abpromise guarantee covers the use of ab20920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. Use with acetone fixed tissues.

Target

  • Relevance
    Staphylococcus aureus are gram-positive bacteria that, although part of the normal flora of humans (nasal passages, skin and mucous membranes), have the potential to become opportunistic pathogens. S. aureus is a frequent cause of a wide range of infections and some bacterial strains have become resistant to many commonly used antibiotics.
  • Alternative names
    • S. aureus antibody

Images

  • ab20920 staining an abscess in rat calf tissue prepared as a 20 µm cryoslice.  Tissue was fixed in paraformaldehyde, permeabilised with 0,5% Trition X100 in PBS, and blocked with 10% serum plus 1% BSA for 1 hour at room temperature.  The primary antibody was diluted 1/500 with PBS plus 3-5% Serum, 0,5% Trition X100 and incubated for 24 hours at 20°C.  An Alexa Fluor® 568 goat anti-rabbit was used as the secondary

    See Abreview

  • ab20920 staining Staphylococcus aureus in human keratinocytes by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in methanol, permeabilized using 0.1% saponin/ PBS, blocked with 4% BSA for 30 minutes at 25°C and then incubated with ab20920 at 10µg/ml for 14 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.

    Red: cadherin
    Blue: DAPI
    Green: anti-Staphylococcus aureus (ab20920)

    See Abreview

References

This product has been referenced in:
  • Hussain S  et al. Antibiotic-loaded nanoparticles targeted to the site of infection enhance antibacterial efficacy. Nat Biomed Eng 2:95-103 (2018). Read more (PubMed: 29955439) »
  • Chung CY  et al. Simultaneous and quantitative monitoring of co-cultured Pseudomonas aeruginosa and Staphylococcus aureus with antibiotics on a diffusometric platform. Sci Rep 7:46336 (2017). Read more (PubMed: 28402317) »
See all 13 Publications for this product

Customer reviews and Q&As

1-10 of 15 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Liver)
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Formaldehyde

Gerhild Rosenberger

Verified customer

Submitted Jan 11 2017

Question
Answer

The Hamza and Li publication BMC Microbiol 14:207 (2014) cites ab20920 as detecting the ATCC 25923 strain so there is evidence it will cross react.

Read More

Question
Answer

The cells used for the immunogen started out as whole cells that were disrupted by sonication and freeze/thaw cycles. Therefore the immunogen is a mixture of intact Staph aureus organisms and soluble proteins from disrupted cells. This is no record of UV-inactivation.

Read More
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Sample
Human Tissue sections (skin (from atopic patient))
Specification
skin (from atopic patient)
Permeabilization
Yes - 0,1% Saponin / PBS
Fixative
Acetone

Abcam user community

Verified customer

Submitted Jul 17 2013

Answer

Thank you very much for your email.
There is absolutely no problem, the antibody will still work properly in my opinion.
Indeed, we have tested different antibodies, and even after a week at 37 degrees Celcius, the antibodies did not seem to loose activity. I am therefore confident that the antibody ab20920 you received, will still work perfectly fine.
If against expectations the antibody would not work, do please contact us again and we will send a replacement vial.
I hope this information is helpful. Please do not hesitate to contact us again, should you have any further question.

Read More

Question
Answer

Thank you for contacting us.

Please find below the information I managed to collect by contacting the sources of the Anti-Staphylococcus aureusand the Anti-Streptococcus agalactiaeantibodies we have in our catalog :

Anti-Staphylococcus aureus:
https://www.abcam.com/ab23568 shows no reactivity to any streptococcus sp., mycoplasma sp. or corynebacteria sp.

Unfortunately, ab35192 has not been specifically tested against streptococcus so we are unable to guarantee it won’t react to S. agalactiae.

ab68950, ab68952, ab68954: These Staphylococcus aureus polyclonal antibodies are not absorbed for related microorganisms and so therefore could cross react with related bacteria such as Staph. Epidermidis.

MLS : Staphylococcus aureus: We do not have an antibody that is specific to Staph. aureus but does not have crossreactivity to Staph. epidermidis.

Anti-Streptococcus agalactiae:
https://www.abcam.com/ab21126 has weak crossreactivity with Strep. dysgalactiae and does not cross-react with Strep. uberis, Strep. bovis, Strep. faecalis, Staph. aureus, Mycoplasma bovis, Mycoplasma californicum, Mycoplasma bovigenitalium, E. coli or Corynebacterium bovis.

I hope this information is helpful to you. I am still waiting for more information about ab128711 (Anti-Streptococcus agalactiae antibody [224/46]).
Please do not hesitate to contact us if you need any more advice or information.

Read More
Application
Immunoprecipitation
Sample
Staphylococcus aureus Cell lysate - whole cell (Primmary Osteoblasts cells)
Total protein in input
200000 cells
Specification
Primmary Osteoblasts cells
Immuno-precipitation step
Other - Dynabeads 280 (invitrogen)

Dr. Lorena Tuchscherr

Verified customer

Submitted Feb 23 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Keratinocytes)
Specification
Keratinocytes
Fixative
Methanol
Permeabilization
Yes - 0,1% Saponin/PBS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 20 2012

Answer

Thank you for contacting us. Indeed, neither ab68954 nor ab20920 have been tested for immunoprecipitation (IP) yet, but they could theoretically be used in this application. If it is just between the two, I would recommend ab20920 because it is an unconjugated antibody. The biotin-labeled antibody ab68954 might not bind to the Protein A or G agarose as well as the unconjugated antibody. Also, ab20920 seems to detect its antigen(s) in a rather native state (as it works with frozen tissue sections) which might be more suitable for IP. However, these antibodies are polyclonal antibodies against various soluble and structural antigens of the whole Staphylococcus aureus. By using the antibodies in IP, a variety of proteins would be pulled down. If this is not appropriate for your experimental setting, I would be pleased to assist you in finding a suitable antibody (for example against a certain target protein). If you decide to go ahead and purchase one of these products though, you might be eligible for our testing discount program. With this, you get a discount off a future purchase if you buy the antibody now, test it in IP and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (abscess in rat calf - cryoslice)
Specification
abscess in rat calf - cryoslice
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,5% Trition X100 in PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Dr. Dagmar Salber

Verified customer

Submitted Jan 09 2008

1-10 of 15 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up