Key features and details
- Rabbit polyclonal to Staphylococcus aureus
- Suitable for: ICC/IF, IHC-Fr
- Reacts with: Staphylococcus aureus
- Isotype: IgG
Product nameAnti-Staphylococcus aureus antibody
See all Staphylococcus aureus primary antibodies
DescriptionRabbit polyclonal to Staphylococcus aureus
SpecificitySoluble and structural antigens of the whole bacterium. Antiserum is not absorbed for related microorganisms.
Tested applicationsSuitable for: ICC/IF, IHC-Frmore details
Species reactivityReacts with: Staphylococcus aureus
Staphylococcus aureus strain ATCC 27660 (mixture of intact organisms and lysed cells).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab20920 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. Use with acetone fixed tissues.|
RelevanceS.aureus binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface, which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
Cellular localizationSecreted; cell wall.
- IgG binding protein A antibody
- Immunoglobulin G binding protein A antibody
- SPA antibody
ab20920 staining an abscess in rat calf tissue prepared as a 20 µm cryoslice. Tissue was fixed in paraformaldehyde, permeabilised with 0,5% Trition X100 in PBS, and blocked with 10% serum plus 1% BSA for 1 hour at room temperature. The primary antibody was diluted 1/500 with PBS plus 3-5% Serum, 0,5% Trition X100 and incubated for 24 hours at 20°C. An Alexa Fluor® 568 goat anti-rabbit was used as the secondary
ab20920 staining Staphylococcus aureus in human keratinocytes by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in methanol, permeabilized using 0.1% saponin/ PBS, blocked with 4% BSA for 30 minutes at 25°C and then incubated with ab20920 at 10µg/ml for 14 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.
Red: cadherinBlue: DAPI
Green: anti-Staphylococcus aureus (ab20920)
ab20920 has been referenced in 18 publications.
- Kubicek-Sutherland JZ et al. Direct detection of bacteremia by exploiting host-pathogen interactions of lipoteichoic acid and lipopolysaccharide. Sci Rep 9:6203 (2019). PubMed: 30996333
- von Gamm M et al. Immune homeostasis and regulation of the interferon pathway require myeloid-derived Regnase-3. J Exp Med 216:1700-1723 (2019). PubMed: 31126966
- Uluçkan Ö et al. Cutaneous Immune Cell-Microbiota Interactions Are Controlled by Epidermal JunB/AP-1. Cell Rep 29:844-859.e3 (2019). PubMed: 31644908
- Hussain S et al. Antibiotic-loaded nanoparticles targeted to the site of infection enhance antibacterial efficacy. Nat Biomed Eng 2:95-103 (2018). PubMed: 29955439
- Chung CY et al. Simultaneous and quantitative monitoring of co-cultured Pseudomonas aeruginosa and Staphylococcus aureus with antibiotics on a diffusometric platform. Sci Rep 7:46336 (2017). PubMed: 28402317