Key features and details
- Chicken polyclonal to Staphylococcus aureus
- Suitable for: WB, ELISA
- Reacts with: Staphylococcus aureus
- Isotype: IgY
Product nameAnti-Staphylococcus aureus antibody
See all Staphylococcus aureus primary antibodies
DescriptionChicken polyclonal to Staphylococcus aureus
SpecificityThis antibody is recognizing both, native and recombinant Protein A, from various sources. Product has not been analyzed strain-specifically.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Staphylococcus aureus
Purified full length recombinant Staphylococcal Protein A
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: 0.9% Sodium chloride
Concentration information loading...
Purification notesThe IgY fraction is isolated by a two step PEG precipitation procedure followed by ammonium sulphate precipitation.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73962 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/5000. Predicted molecular weight: 60 kDa.
One precipitin arc in immunodiffusion against Staphylococcal Protein A.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceS.aureus binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface, which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
Cellular localizationSecreted; cell wall.
- IgG binding protein A antibody
- Immunoglobulin G binding protein A antibody
- SPA antibody
ab73962 has been referenced in 1 publication.
- Veli M & Ozcan A Computational Sensing of Staphylococcus aureus on Contact Lenses Using 3D Imaging of Curved Surfaces and Machine Learning. ACS Nano 12:2554-2559 (2018). PubMed: 29522316