Overview

  • Product name

    Anti-Staphylococcus TSST1 antibody (HRP)
    See all Staphylococcus TSST1 primary antibodies
  • Description

    Rabbit polyclonal to Staphylococcus TSST1 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Specificity

    Absorbance readings (410 nm) of less than 0.100 for 10 ng/ml preparations of staphylococcal enterotoxins B through E, ET, and alpha hemolysin.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Staphylococcus aureus
  • Immunogen

    Purified toxin (>95% pure).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: 1/300.
    This is an approximate working dilution for use in a sandwich ELISA. This dilution will give an absorbance of >1.0 for a 10 ng standard after a 5 minute incubation.
    WB: 1/1000. Predicted molecular weight: 28 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      This secreted toxin is responsible for the symptoms of toxic shock syndrome. It belongs to the staphylococcal/streptococcal toxin family.
    • Cellular localization

      Secreted
    • Database links

      • Alternative names

        • Toxic shock syndrome toxin 1 antibody
        • TSST1 antibody
        • Tst antibody

      References

      ab15934 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      1-10 of 10 Abreviews or Q&A

      Question
      Answer

      Thank you for contacting us.

      Please find attached the requested CoC.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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      Answer

      Thank you for contacting us.

      We have updated the datasheet for ab15934 on our website to include a pdf of the Material safety data sheet. You can find it by accessing the web page below:

      https://www.abcam.com/ps/Products/15/ab15934/Msds/ab15934_CLP1_EN.pdf

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Answer

      Thank you for contacting us.

      Unfortunately, I will not be able to disclose the information. This is developed by our collaborator supplier. Any information related to the collaborator is proprietary however we can make this available or you if you are interested. I am very for any inconvenience this may have caused.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

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      Answer

      Thank you for contacting us.

      The IgG-conjugate complex is stabilized with Bovine Serum Albumin (BSA), to prevent denaturization during lyophilization. The BSA and horseradish peroxidase enzyme will give an elevated total protein concentration. The conjugate is tested using a standard sandwich ELISA (anti-TSST-1
      IgG , TSST-1, IgG-conjugate and substrate).

      The conjugate was tested using TSST-1 serially diluted from 10ng down to 0.31ng. The absorbance of the 1.25ng TSST-1 standard must be greater than 0.200 when read at 405nM after a 15 minute incubation (when the conjugate is diluted 1:300).

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Answer

      Thank you for contacting us.
      I have discussed this issue with lab; they however are interested in seeing raw data; would it be possible if you could send the data to us? Please be assured that we do not publish any data, other than customer's inquiries.

      I will look forward to hearing from you soon.

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      Answer

      Thank you for contacting us and your interest in our products.

      With the anti-Staphylococcus TSST1 antibody (HRP) (ab15934) we would advise storing the antibody short term as +4°C (1-2 weeks) and for longer term, aliquotted at -20°C or -80°C. We have not performed further stability tests with this particular antibody, I cannot therefore say whether the antibody is likely to have maintained its activity when stored at 4°C for2 months. I would advise trying the antibody in your tests to see how it performs. We often find that antibodies are stable at if stored at 4°C for up to a year but as I have mentioned, I would not be able to guarantee this.

      I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

      Read More

      Answer

      I apologize for misinterpretation of conc. I have received an updated reply from lab.

      The 250ul volume you received should have the conc. of 1.5 mg/ml. (the 0.75 mg product was dissolved in 500 ul of deonized water before shipping)

      I hope this will help.

      Read More

      Answer

      Just to let you know I am still waiting for a confirmation from my colleague. I will send you the details soon. Many thanks for having patience.

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      Answer

      I am waiting for more information from lab to clarify this. I will send you the details soon.

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      Question
      Answer

      Thank you for contacting us.

      The conc of current lot GR40466-1 is approximately 0.75 mg. The conc is lot specific, please contact us for conc of other lots.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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