Overview

  • Product name

  • Description

    Rabbit polyclonal to StAR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human StAR aa 130-180 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Sequence:

    M ERLYEELVER MEAMGEWNPN VKEIKVLQKI GKDTFITHEL AAEAAGNLVG


    Database link: P49675

  • Positive control

    • Rat kidney and mouse embryo tissues. Mouse brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab203193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use at 1/50 - 1/200 with fluorescent detection methods.

WB 1/100 - 1/1000. Predicted molecular weight: 32 kDa.

Target

  • Function

    Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.
  • Tissue specificity

    Expressed in gonads, adrenal cortex and kidney.
  • Pathway

    Steroid metabolism; cholesterol metabolism.
  • Involvement in disease

    Defects in STAR are the cause of adrenal hyperplasia type 1 (AH1) [MIM:201710]. The most severe form of adrenal hyperplasia. It is a condition characterized by onset of profound adrenocortical insufficiency shortly after birth, hyperpigmentation reflecting increased production of pro-opiomelanocortin, elevated plasma renin activity as a consequence of reduced aldosterone synthesis, and male pseudohermaphroditism resulting from deficient fetal testicular testosterone synthesis. Affected individuals are phenotypic females irrespective of gonadal sex, and frequently die in infancy if mineralocorticoid and glucocorticoid replacement are not instituted.
  • Sequence similarities

    Contains 1 START domain.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cholesterol trafficker antibody
    • Luteinizing hormone induced protein antibody
    • mitochondrial antibody
    • Mitochondrial steroid acute regulatory protein antibody
    • StAR antibody
    • StAR related lipid transfer (START) domain containing 1 antibody
    • STAR_HUMAN antibody
    • StARD1 antibody
    • START domain containing 1 antibody
    • START domain containing protein 1 antibody
    • START domain-containing protein 1 antibody
    • Steroid acute regulatory protein antibody
    • Steroidogenic acute regulator antibody
    • Steroidogenic acute regulatory protein antibody
    • Steroidogenic acute regulatory protein mitochondrial antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded rat kidney tissue, labeling StAR using ab203193 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded mouse embryo tissue, labeling StAR using ab203193 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

References

This product has been referenced in:

  • Huang X  et al. Effects of electroacupuncture on luteal regression and steroidogenesis in ovarian hyperstimulation syndrome model rat. Life Sci 197:1-9 (2018). WB . Read more (PubMed: 29482802) »
  • Chung HJ  et al. Steroidogenic effects of Taraxacum officinale extract on the levels of steroidogenic enzymes in mouse Leydig cells. Anim Cells Syst (Seoul) 22:407-414 (2018). Read more (PubMed: 30533263) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Rat Cell lysate - whole cell (Epithelial Cells)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
75 µg
Specification
Epithelial Cells
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Nov 11 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Gonad)
Antigen retrieval step
Enzymatic
Permeabilization
No
Specification
Gonad
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 30 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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