Overview

  • Product name

  • Description

    Mouse monoclonal to StAR
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISA, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment: EAMQKALGIL SNQEGWKKES QQDNGDKVMS KVVPDVGKVF RLEVVVDQPM ERLYEELVER MEAMGEWNPN VKEIKVLQKI GKDTFITHEL AAEAAGNLVG , corresponding to amino acids 81-180 of Human StAR

  • General notes

    This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Applications

Our Abpromise guarantee covers the use of ab58013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 21094146

Target

  • Function

    Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.
  • Tissue specificity

    Expressed in gonads, adrenal cortex and kidney.
  • Pathway

    Steroid metabolism; cholesterol metabolism.
  • Involvement in disease

    Defects in STAR are the cause of adrenal hyperplasia type 1 (AH1) [MIM:201710]. The most severe form of adrenal hyperplasia. It is a condition characterized by onset of profound adrenocortical insufficiency shortly after birth, hyperpigmentation reflecting increased production of pro-opiomelanocortin, elevated plasma renin activity as a consequence of reduced aldosterone synthesis, and male pseudohermaphroditism resulting from deficient fetal testicular testosterone synthesis. Affected individuals are phenotypic females irrespective of gonadal sex, and frequently die in infancy if mineralocorticoid and glucocorticoid replacement are not instituted.
  • Sequence similarities

    Contains 1 START domain.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cholesterol trafficker antibody
    • Luteinizing hormone induced protein antibody
    • mitochondrial antibody
    • Mitochondrial steroid acute regulatory protein antibody
    • StAR antibody
    • StAR related lipid transfer (START) domain containing 1 antibody
    • STAR_HUMAN antibody
    • StARD1 antibody
    • START domain containing 1 antibody
    • START domain containing protein 1 antibody
    • START domain-containing protein 1 antibody
    • Steroid acute regulatory protein antibody
    • Steroidogenic acute regulator antibody
    • Steroidogenic acute regulatory protein antibody
    • Steroidogenic acute regulatory protein mitochondrial antibody
    see all

Images

  • Anti-StAR antibody (ab58013) at 1/1000 dilution + Mouse liver lysates at 50 µg

    Secondary
    HRP-conjugated Goat polyclonal to mouse IgG at 1/2500 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 27,30 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 minutes


    Liver lysates prepared from mouse injected with StarD1 adenovirus expressing recombinant StarD1 protein.

    Primary diluted in TBST + 2.5% milk and incubated with sample for 12 hours at 4°C.

    This image was generated using the ascites version of the product.

  • Anti-StAR antibody (ab58013) at 1/1000 dilution (in TBST + 2.5% milk for 16 hours at 4°C) + Whole cell lysate of human THP1 cells at 50 µg

    Secondary
    An HRP-conjugated Goat anti-mouse IgG polyclonal at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 30 kDa why is the actual band size different from the predicted?


    Exposure time: 2 minutes


    Blocking Step: 5% Milk for 4 hours at 22°C

    This image was generated using the ascites version of the product.

    See Abreview

References

This product has been referenced in:

  • Meimaridou E  et al. NNT is a key regulator of adrenal redox homeostasis and steroidogenesis in male mice. J Endocrinol 236:13-28 (2018). Read more (PubMed: 29046340) »
  • Hou X  et al. Pubertal chlorocholine chloride exposure inhibits testicular testosterone synthesis by down-regulating steroidogenic enzymes in adult rats. Toxicol Lett 288:17-24 (2018). WB ; Rat . Read more (PubMed: 29447956) »
See all 11 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

1-8 of 8 Abreviews

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (adrenal tissue)
Permeabilization
Yes - 0.1 triton
Specification
adrenal tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4µg/mL · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted May 29 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary rat cardiac fibroblasts)
Permeabilization
Yes - 1% Triton, 4 minutes
Specification
Primary rat cardiac fibroblasts
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 20 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human adrenal corticocarcinoma H295R cells)
Permeabilization
Yes - 0.5% TX100
Specification
Human adrenal corticocarcinoma H295R cells
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Jun 27 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (Human adrenal corticocarcinoma H295R cells)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
25 µg
Specification
Human adrenal corticocarcinoma H295R cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Jun 27 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse testis)
Permeabilization
Yes - 0.5% TX100
Specification
mouse testis
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Dec 02 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1 cells)
Loading amount
50 µg
Specification
THP-1 cells
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Sep 22 2010

Application
Western blot
Sample
Rat Cell lysate - whole cell (testis)
Loading amount
35 µg
Specification
testis
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 22°C

Abcam user community

Verified customer

Submitted Feb 09 2010

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 20 2009

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up