Key features and details
- Rabbit polyclonal to StAR
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-StAR antibody
See all StAR primary antibodies
DescriptionRabbit polyclonal to StAR
Tested applicationsSuitable for: ICC, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Dog, Pig
Recombinant protein fragment corresponding to a region within amino acids 15 - 238 of Human StAR (NP_000340).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab96637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|WB||1/100 - 1/10000. Predicted molecular weight: 32 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionPlays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.
Tissue specificityExpressed in gonads, adrenal cortex and kidney.
PathwaySteroid metabolism; cholesterol metabolism.
Involvement in diseaseDefects in STAR are the cause of adrenal hyperplasia type 1 (AH1) [MIM:201710]. The most severe form of adrenal hyperplasia. It is a condition characterized by onset of profound adrenocortical insufficiency shortly after birth, hyperpigmentation reflecting increased production of pro-opiomelanocortin, elevated plasma renin activity as a consequence of reduced aldosterone synthesis, and male pseudohermaphroditism resulting from deficient fetal testicular testosterone synthesis. Affected individuals are phenotypic females irrespective of gonadal sex, and frequently die in infancy if mineralocorticoid and glucocorticoid replacement are not instituted.
Sequence similaritiesContains 1 START domain.
- Information by UniProt
- Cholesterol trafficker antibody
- Luteinizing hormone induced protein antibody
- mitochondrial antibody
Anti-StAR antibody (ab96637) at 1/1000 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate at 30 µg
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 32 kDa
Immunohistochemical analysis of paraffin-embedded human hepatoma tissue staining StAR protein at cytosol with ab96637 at 1/500.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
Immunofluorescence analysis of 4% paraformaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate labeling StAR protein at mitochondria with ab96637 at 1/500 dilution. Blue: Hoechst 33342 staining.
All lanes : Anti-StAR antibody (ab96637) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 32 kDa
All lanes : Anti-StAR antibody (ab96637) at 1/1000 dilution
Lane 1 : Whole tissue lysate prepared from murine adrenal gland
Lane 2 : Whole tissue lysate prepared from rat adrenal gland
Lysates/proteins at 50 µg per lane.
All lanes : HRP conjugated goat anti-rabbit polyclonal at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 32 kDa
Exposure time: 20 seconds
ab96637 has been referenced in 12 publications.
- Plewes MR et al. Yes-associated protein 1 is required for proliferation and function of bovine granulosa cells in vitro†. Biol Reprod 101:1001-1017 (2019). PubMed: 31350850
- Shi X & Fu L Piceatannol inhibits oxidative stress through modification of Nrf2-signaling pathway in testes and attenuates spermatogenesis and steroidogenesis in rats exposed to cadmium during adulthood. Drug Des Devel Ther 13:2811-2824 (2019). PubMed: 31496657
- Gao X et al. Prenatal exposure to zearalenone disrupts reproductive potential and development via hormone-related genes in male rats. Food Chem Toxicol 116:11-19 (2018). WB . PubMed: 29627501
- Ronis MJ et al. EB 2017 Article: Soy protein isolate feeding does not result in reproductive toxicity in the pre-pubertal rat testis. Exp Biol Med (Maywood) 243:695-707 (2018). PubMed: 29763383
- Fadhillah et al. Hypoxia-inducible factor 1 mediates hypoxia-enhanced synthesis of progesterone during luteinization of granulosa cells. J Reprod Dev 63:75-85 (2017). WB ; Cow . PubMed: 27840375