Overview

  • Product name

  • Description

    Rabbit polyclonal to StAR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog, Pig
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 15 - 238 of Human StAR (NP_000340).

  • Positive control

    • 293T, A431, HeLa, HepG2, MOLT4 and Raji cell lysates

Applications

Our Abpromise guarantee covers the use of ab96637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 32 kDa.

Target

  • Function

    Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.
  • Tissue specificity

    Expressed in gonads, adrenal cortex and kidney.
  • Pathway

    Steroid metabolism; cholesterol metabolism.
  • Involvement in disease

    Defects in STAR are the cause of adrenal hyperplasia type 1 (AH1) [MIM:201710]. The most severe form of adrenal hyperplasia. It is a condition characterized by onset of profound adrenocortical insufficiency shortly after birth, hyperpigmentation reflecting increased production of pro-opiomelanocortin, elevated plasma renin activity as a consequence of reduced aldosterone synthesis, and male pseudohermaphroditism resulting from deficient fetal testicular testosterone synthesis. Affected individuals are phenotypic females irrespective of gonadal sex, and frequently die in infancy if mineralocorticoid and glucocorticoid replacement are not instituted.
  • Sequence similarities

    Contains 1 START domain.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cholesterol trafficker antibody
    • Luteinizing hormone induced protein antibody
    • mitochondrial antibody
    • Mitochondrial steroid acute regulatory protein antibody
    • StAR antibody
    • StAR related lipid transfer (START) domain containing 1 antibody
    • STAR_HUMAN antibody
    • StARD1 antibody
    • START domain containing 1 antibody
    • START domain containing protein 1 antibody
    • START domain-containing protein 1 antibody
    • Steroid acute regulatory protein antibody
    • Steroidogenic acute regulator antibody
    • Steroidogenic acute regulatory protein antibody
    • Steroidogenic acute regulatory protein mitochondrial antibody
    see all

Images

  • Anti-StAR antibody (ab96637) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 32 kDa



    12% SDS PAGE
  • All lanes : Anti-StAR antibody (ab96637) at 1/1000 dilution

    Lane 1 : Whole tissue lysate prepared from murine adrenal gland
    Lane 2 : Whole tissue lysate prepared from rat adrenal gland

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rabbit polyclonal at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 32 kDa


    Exposure time: 20 seconds

    See Abreview

References

This product has been referenced in:

  • Gao X  et al. Prenatal exposure to zearalenone disrupts reproductive potential and development via hormone-related genes in male rats. Food Chem Toxicol 116:11-19 (2018). WB . Read more (PubMed: 29627501) »
  • Ronis MJ  et al. EB 2017 Article: Soy protein isolate feeding does not result in reproductive toxicity in the pre-pubertal rat testis. Exp Biol Med (Maywood) 243:695-707 (2018). Read more (PubMed: 29763383) »
See all 9 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Question
Answer

Thank you very much for your interest in ab96637. Sinceab96637 has not been tested in pig, I can offer a discount off a future purchase if you buy ab96637 now, test it in pig, and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be worth 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab96637 in pig. I will then send a discount code. This code must be issued before purchasing ab96637 so please wait for my reply before ordering. 2. Purchase ab96637 either by phone, fax, or online (www.abcam.com). 3. Test it in pig. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab96637 turns out to be unsuitable for pig, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Question

DESCRIPTION OF THE PROBLEM Non-specific staining Staining pattern is absent in the paraffin embedded sections of mice testis SAMPLE Fresh testis Bouin’s / Davidson’s fixed paraffin embedded sections. PRIMARY ANTIBODY Concentration or dilution 1:25. 1:50 and 1:100 Diluent buffer 1% BSA in PBS Incubation time overnight Incubation temperature 4 ° C DETECTION METHOD hrp POSITIVE AND NEGATIVE CONTROLS USED Positive control- Anti- P450 SCC antibody, Rabbit polyclonal (1:100) ANTIBODY STORAGE CONDITIONS 4 degC FIXATION OF SAMPLE Fixation: Yes/No Not necessary Fixative agent and concentration Fixation time Fixation temperature ANTIGEN RETRIEVAL no PERMEABILIZATION STEP Yes/No Not necessary Permeabilization agent and concentration Permeabilization time Permeabilization temperature BLOCKING CONDITIONS Yes/No Blocking agent and concentration 15% FBS Blocking time 30 minutes Blocking temperature RT Endogenous peroxidases blocked? Yes/No yes Endogenous biotins blocked? Yes/No no SECONDARY ANTIBODY Concentration or dilution 1:25. 1:50 and 1:100 Diluent buffer 1% BSA in PBS Incubation time overnight Incubation temperature 4 ° C Species Goat Anti-rabbit IgG Reacts against rabbit Concentration or dilution 1:100 Diluent buffer in PBS Incubation time I hour Incubation temperature: 37° celsius Fluorochrome or enzyme conjugate Enzyme conjugate HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Concentration of primary antibody ADDITIONAL NOTES After primary antibody After secondary antibody Buffer Buffer pbs Buffer pbs Number of washes 3 Number of washes Buffer 3

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Answer

Thank you for submitting your completed questionnaire. To our knowledge ab96637 has not been previously tested in IHC-P. Thus is may be that this antibody does not function effectively in this application. However, results may be improved by doing an antigen retrieval step, for example a heat induced epitope retreival with pH 9.0 buffer. An example of this protocol, along with a recipe for this retrieval buffer can be found in our IHC protocol: https://www.abcam.com/index.html?pageconfig=resource&rid=11384 I hope this is helpful. I look forward to hearing if this tip proves useful. Please contact me again if you have any further questions.

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Application
Western blot
Sample
Rat Tissue lysate - whole (Adrenal)
Loading amount
50 µg
Specification
Adrenal
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Aug 23 2011

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Oct 20 2010

Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1)
Loading amount
25 µg
Specification
THP-1
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Sep 29 2010

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