Overview

  • Product name

  • Description

    Rabbit polyclonal to StAR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog, Pig
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 15 - 238 of Human StAR (NP_000340).

  • Positive control

    • 293T, A431, HeLa, HepG2, MOLT4 and Raji cell lysates

Applications

Our Abpromise guarantee covers the use of ab96637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 32 kDa.

Target

  • Function

    Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.
  • Tissue specificity

    Expressed in gonads, adrenal cortex and kidney.
  • Pathway

    Steroid metabolism; cholesterol metabolism.
  • Involvement in disease

    Defects in STAR are the cause of adrenal hyperplasia type 1 (AH1) [MIM:201710]. The most severe form of adrenal hyperplasia. It is a condition characterized by onset of profound adrenocortical insufficiency shortly after birth, hyperpigmentation reflecting increased production of pro-opiomelanocortin, elevated plasma renin activity as a consequence of reduced aldosterone synthesis, and male pseudohermaphroditism resulting from deficient fetal testicular testosterone synthesis. Affected individuals are phenotypic females irrespective of gonadal sex, and frequently die in infancy if mineralocorticoid and glucocorticoid replacement are not instituted.
  • Sequence similarities

    Contains 1 START domain.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cholesterol trafficker antibody
    • Luteinizing hormone induced protein antibody
    • mitochondrial antibody
    • Mitochondrial steroid acute regulatory protein antibody
    • StAR antibody
    • StAR related lipid transfer (START) domain containing 1 antibody
    • STAR_HUMAN antibody
    • StARD1 antibody
    • START domain containing 1 antibody
    • START domain containing protein 1 antibody
    • START domain-containing protein 1 antibody
    • Steroid acute regulatory protein antibody
    • Steroidogenic acute regulator antibody
    • Steroidogenic acute regulatory protein antibody
    • Steroidogenic acute regulatory protein mitochondrial antibody
    see all

Images

  • Anti-StAR antibody (ab96637) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 32 kDa



    12% SDS PAGE
  • All lanes : Anti-StAR antibody (ab96637) at 1/1000 dilution

    Lane 1 : Whole tissue lysate prepared from murine adrenal gland
    Lane 2 : Whole tissue lysate prepared from rat adrenal gland

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rabbit polyclonal at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 32 kDa


    Exposure time: 20 seconds

    See Abreview

References

This product has been referenced in:

  • Gao X  et al. Prenatal exposure to zearalenone disrupts reproductive potential and development via hormone-related genes in male rats. Food Chem Toxicol 116:11-19 (2018). WB . Read more (PubMed: 29627501) »
  • Ronis MJ  et al. EB 2017 Article: Soy protein isolate feeding does not result in reproductive toxicity in the pre-pubertal rat testis. Exp Biol Med (Maywood) 243:695-707 (2018). Read more (PubMed: 29763383) »
See all 9 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

1-3 of 3 Abreviews

Application
Western blot
Sample
Rat Tissue lysate - whole (Adrenal)
Loading amount
50 µg
Specification
Adrenal
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Aug 23 2011

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Oct 20 2010

Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1)
Loading amount
25 µg
Specification
THP-1
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Sep 29 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up