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RecombinantRabMAb

Recombinant Anti-StAR antibody [EP7639] (ab133657)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (6)

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Western blot - Anti-StAR antibody [EP7639] (ab133657)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)
  • Anti-StAR antibody [EP7639] (ab133657)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP7639] to StAR
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-StAR antibody [EP7639]
    See all StAR primary antibodies
  • Description

    Rabbit monoclonal [EP7639] to StAR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human StAR aa 250 to the C-terminus. The exact sequence is proprietary.

  • Positive control

    • Human adrenal gland lysate; Human adrenal gland tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP7639
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis

Associated products

  • Alternative Versions

    • Anti-StAR antibody [EP7639] - BSA and Azide free (ab244236)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human StAR protein (ab115707)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/10000 - 1/50000. Detects a band of approximately 30 kDa (predicted molecular weight: 32 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/10000 - 1/50000. Detects a band of approximately 30 kDa (predicted molecular weight: 32 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.
    • Tissue specificity

      Expressed in gonads, adrenal cortex and kidney.
    • Pathway

      Steroid metabolism; cholesterol metabolism.
    • Involvement in disease

      Defects in STAR are the cause of adrenal hyperplasia type 1 (AH1) [MIM:201710]. The most severe form of adrenal hyperplasia. It is a condition characterized by onset of profound adrenocortical insufficiency shortly after birth, hyperpigmentation reflecting increased production of pro-opiomelanocortin, elevated plasma renin activity as a consequence of reduced aldosterone synthesis, and male pseudohermaphroditism resulting from deficient fetal testicular testosterone synthesis. Affected individuals are phenotypic females irrespective of gonadal sex, and frequently die in infancy if mineralocorticoid and glucocorticoid replacement are not instituted.
    • Sequence similarities

      Contains 1 START domain.
    • Cellular localization

      Mitochondrion.
    • Target information above from: UniProt accession P49675 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6770 Human
      • Omim: 600617 Human
      • SwissProt: P49675 Human
      • Unigene: 521535 Human
      • Alternative names

        • Cholesterol trafficker antibody
        • Luteinizing hormone induced protein antibody
        • mitochondrial antibody
        • Mitochondrial steroid acute regulatory protein antibody
        • StAR antibody
        • StAR related lipid transfer (START) domain containing 1 antibody
        • STAR_HUMAN antibody
        • StARD1 antibody
        • START domain containing 1 antibody
        • START domain containing protein 1 antibody
        • START domain-containing protein 1 antibody
        • Steroid acute regulatory protein antibody
        • Steroidogenic acute regulator antibody
        • Steroidogenic acute regulatory protein antibody
        • Steroidogenic acute regulatory protein mitochondrial antibody
        see all

      Images

      • Western blot - Anti-StAR antibody [EP7639] (ab133657)
        Western blot - Anti-StAR antibody [EP7639] (ab133657)
        Anti-StAR antibody [EP7639] (ab133657) at 1/10000 dilution + Human adrenal gland lysate at 10 µg

        Secondary
        HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 32 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)

        Immunohistochemical analysis of paraffin-embedded Human adrenal gland tissue labelling StAR with ab133657 at 1/250 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)

        ab133657 showing positive staining in Normal testis tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)

        ab133657 showing positive staining in Adrenocortical carcinoma tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)

        ab133657 showing negative staining in Skeletal muscle tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)

        ab133657 showing positive staining in Sertoli-Leydig cell tumor tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Anti-StAR antibody [EP7639] (ab133657)
        Anti-StAR antibody [EP7639] (ab133657)

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (6)

      Publishing research using ab133657? Please let us know so that we can cite the reference in this datasheet.

      ab133657 has been referenced in 6 publications.

      • Asiabi P  et al. Immunodetection and quantification of enzymatic markers in theca cells: the early process of ovarian steroidogenesis†. Biol Reprod 102:145-155 (2020). PubMed: 31504196
      • Manna PR  et al. Overexpression of the steroidogenic acute regulatory protein in breast cancer: Regulation by histone deacetylase inhibition. Biochem Biophys Res Commun 509:476-482 (2019). PubMed: 30595381
      • Chen Y  et al. Differentiation of human adipose derived stem cells into Leydig-like cells with molecular compounds. J Cell Mol Med 23:5956-5969 (2019). PubMed: 31293077
      • Bai J  et al. Synthesis and biological evaluation of methylpyrimidine-fused tricyclic diterpene analogs as novel oral anti-late-onset hypogonadism agents. Eur J Med Chem 176:21-40 (2019). PubMed: 31091478
      • Ide H  et al. Modulation of AKR1C2 by curcumin decreases testosterone production in prostate cancer. Cancer Sci 109:1230-1238 (2018). WB ; Human . PubMed: 29369461
      • Guo X  et al. Induced pluripotent stem cell-derived conditional medium promotes Leydig cell anti-apoptosis and proliferation via autophagy and Wnt/ß-catenin pathway. J Cell Mol Med 22:3614-3626 (2018). PubMed: 29667777

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Western blot abreview for Anti-StAR antibody [EP7639]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Human adrenal corticocarcinoma H295R cells)
      Gel Running Conditions
      Reduced Denaturing (15)
      Loading amount
      25 µg
      Specification
      Human adrenal corticocarcinoma H295R cells
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Herr Dr. Vladimir Milenkovic

      Verified customer

      Submitted Jun 27 2017

      Western blot abreview for Anti-StAR antibody [EP7639]

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Sheep Tissue lysate - whole (ovine theca cells, ov-placenta tissue, ov-liver ti)
      Gel Running Conditions
      Reduced Denaturing (10% PAG)
      Loading amount
      20 µg
      Specification
      ovine theca cells, ov-placenta tissue, ov-liver ti
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Read More

      Miss. Barbara Makela

      Verified customer

      Submitted Jun 23 2017

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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