Name: Anti-StAR antibody [EP7639]
Brand: RabMAb
SKU: ab133657
Rating: 4 (2 reviews)

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Western blot - Anti-StAR antibody [EP7639] (ab133657)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP7639] to StAR
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-StAR antibody [EP7639]
See all StAR primary antibodies
Description

Rabbit monoclonal [EP7639] to StAR
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Unsuitable for: IP
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide within Human StAR aa 250 to the C-terminus. The exact sequence is proprietary.
Positive control
- Human adrenal gland lysate; Human adrenal gland tissue.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP7639
Isotype

IgG
Research areas

Alternative Versions
- Anti-StAR antibody [EP7639] - BSA and Azide free (ab244236)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human StAR protein (ab115707)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(2)	1/10000 - 1/50000. Detects a band of approximately 30 kDa (predicted molecular weight: 32 kDa).
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Notes
WB 1/10000 - 1/50000. Detects a band of approximately 30 kDa (predicted molecular weight: 32 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Application notes

Is unsuitable for IP.

Function

Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.
Tissue specificity

Expressed in gonads, adrenal cortex and kidney.
Pathway

Steroid metabolism; cholesterol metabolism.
Involvement in disease

Defects in STAR are the cause of adrenal hyperplasia type 1 (AH1) [MIM:201710]. The most severe form of adrenal hyperplasia. It is a condition characterized by onset of profound adrenocortical insufficiency shortly after birth, hyperpigmentation reflecting increased production of pro-opiomelanocortin, elevated plasma renin activity as a consequence of reduced aldosterone synthesis, and male pseudohermaphroditism resulting from deficient fetal testicular testosterone synthesis. Affected individuals are phenotypic females irrespective of gonadal sex, and frequently die in infancy if mineralocorticoid and glucocorticoid replacement are not instituted.
Sequence similarities

Contains 1 START domain.
Cellular localization

Mitochondrion.
Target information above from: UniProt accession P49675 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 6770 Human
- Omim: 600617 Human
- SwissProt: P49675 Human
- Unigene: 521535 Human
Alternative names
- Cholesterol trafficker antibody
- Luteinizing hormone induced protein antibody
- mitochondrial antibody
- Mitochondrial steroid acute regulatory protein antibody
- StAR antibody
- StAR related lipid transfer (START) domain containing 1 antibody
- STAR_HUMAN antibody
- StARD1 antibody
- START domain containing 1 antibody
- START domain containing protein 1 antibody
- START domain-containing protein 1 antibody
- Steroid acute regulatory protein antibody
- Steroidogenic acute regulator antibody
- Steroidogenic acute regulatory protein antibody
- Steroidogenic acute regulatory protein mitochondrial antibody
see all

Western blot - Anti-StAR antibody [EP7639] (ab133657)

Anti-StAR antibody [EP7639] (ab133657) at 1/10000 dilution + Human adrenal gland lysate at 10 µg

Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)

Immunohistochemical analysis of paraffin-embedded Human adrenal gland tissue labelling StAR with ab133657 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)

ab133657 showing positive staining in Normal testis tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)

ab133657 showing positive staining in Adrenocortical carcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)

ab133657 showing negative staining in Skeletal muscle tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)

ab133657 showing positive staining in Sertoli-Leydig cell tumor tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-StAR antibody [EP7639] (ab133657)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab133657? Please let us know so that we can cite the reference in this datasheet.

ab133657 has been referenced in 6 publications.

Asiabi P et al. Immunodetection and quantification of enzymatic markers in theca cells: the early process of ovarian steroidogenesis†. Biol Reprod 102:145-155 (2020). PubMed: 31504196
Manna PR et al. Overexpression of the steroidogenic acute regulatory protein in breast cancer: Regulation by histone deacetylase inhibition. Biochem Biophys Res Commun 509:476-482 (2019). PubMed: 30595381
Chen Y et al. Differentiation of human adipose derived stem cells into Leydig-like cells with molecular compounds. J Cell Mol Med 23:5956-5969 (2019). PubMed: 31293077
Bai J et al. Synthesis and biological evaluation of methylpyrimidine-fused tricyclic diterpene analogs as novel oral anti-late-onset hypogonadism agents. Eur J Med Chem 176:21-40 (2019). PubMed: 31091478
Ide H et al. Modulation of AKR1C2 by curcumin decreases testosterone production in prostate cancer. Cancer Sci 109:1230-1238 (2018). WB ; Human . PubMed: 29369461
Guo X et al. Induced pluripotent stem cell-derived conditional medium promotes Leydig cell anti-apoptosis and proliferation via autophagy and Wnt/ß-catenin pathway. J Cell Mol Med 22:3614-3626 (2018). PubMed: 29667777

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (Human adrenal corticocarcinoma H295R cells)

Gel Running Conditions

Reduced Denaturing (15)

Loading amount

25 µg

Specification

Human adrenal corticocarcinoma H295R cells

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Jun 27 2017

Application

Western blot

Sample

Sheep Tissue lysate - whole (ovine theca cells, ov-placenta tissue, ov-liver ti)

Gel Running Conditions

Reduced Denaturing (10% PAG)

Loading amount

20 µg

Specification

ovine theca cells, ov-placenta tissue, ov-liver ti

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Miss. Barbara Makela

Verified customer

Submitted Jun 23 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Pathway

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (6)

Customer reviews and Q&As

Western blot abreview for Anti-StAR antibody [EP7639]

Western blot abreview for Anti-StAR antibody [EP7639]