Overview

  • Product name

    Anti-StAR antibody [EP7639]
    See all StAR primary antibodies
  • Description

    Rabbit monoclonal [EP7639] to StAR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human StAR aa 250-350. The exact sequence is proprietary.

  • Positive control

    • Human adrenal gland lysate; Human adrenal gland tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 30 kDa (predicted molecular weight: 32 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.
    • Tissue specificity

      Expressed in gonads, adrenal cortex and kidney.
    • Pathway

      Steroid metabolism; cholesterol metabolism.
    • Involvement in disease

      Defects in STAR are the cause of adrenal hyperplasia type 1 (AH1) [MIM:201710]. The most severe form of adrenal hyperplasia. It is a condition characterized by onset of profound adrenocortical insufficiency shortly after birth, hyperpigmentation reflecting increased production of pro-opiomelanocortin, elevated plasma renin activity as a consequence of reduced aldosterone synthesis, and male pseudohermaphroditism resulting from deficient fetal testicular testosterone synthesis. Affected individuals are phenotypic females irrespective of gonadal sex, and frequently die in infancy if mineralocorticoid and glucocorticoid replacement are not instituted.
    • Sequence similarities

      Contains 1 START domain.
    • Cellular localization

      Mitochondrion.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cholesterol trafficker antibody
      • Luteinizing hormone induced protein antibody
      • mitochondrial antibody
      • Mitochondrial steroid acute regulatory protein antibody
      • StAR antibody
      • StAR related lipid transfer (START) domain containing 1 antibody
      • STAR_HUMAN antibody
      • StARD1 antibody
      • START domain containing 1 antibody
      • START domain containing protein 1 antibody
      • START domain-containing protein 1 antibody
      • Steroid acute regulatory protein antibody
      • Steroidogenic acute regulator antibody
      • Steroidogenic acute regulatory protein antibody
      • Steroidogenic acute regulatory protein mitochondrial antibody
      see all

    Images

    • Anti-StAR antibody [EP7639] (ab133657) at 1/10000 dilution + Human adrenal gland lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 32 kDa

    • Immunohistochemical analysis of paraffin-embedded Human adrenal gland tissue labelling StAR with ab133657 at 1/250 dilution.
    • ab133657 showing positive staining in Normal testis tissue.

    • ab133657 showing positive staining in Adrenocortical carcinoma tissue.

    • ab133657 showing negative staining in Skeletal muscle tissue.

    • ab133657 showing positive staining in Sertoli-Leydig cell tumor tissue.

    References

    This product has been referenced in:

    • Manna PR  et al. Overexpression of the steroidogenic acute regulatory protein in breast cancer: Regulation by histone deacetylase inhibition. Biochem Biophys Res Commun 509:476-482 (2019). Read more (PubMed: 30595381) »
    • Ide H  et al. Modulation of AKR1C2 by curcumin decreases testosterone production in prostate cancer. Cancer Sci 109:1230-1238 (2018). WB ; Human . Read more (PubMed: 29369461) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human adrenal corticocarcinoma H295R cells)
    Gel Running Conditions
    Reduced Denaturing (15)
    Loading amount
    25 µg
    Specification
    Human adrenal corticocarcinoma H295R cells
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

    Herr Dr. Vladimir Milenkovic

    Verified customer

    Submitted Jun 27 2017

    Application
    Western blot
    Sample
    Sheep Tissue lysate - whole (ovine theca cells, ov-placenta tissue, ov-liver ti)
    Gel Running Conditions
    Reduced Denaturing (10% PAG)
    Loading amount
    20 µg
    Specification
    ovine theca cells, ov-placenta tissue, ov-liver ti
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

    Miss. Barbara Makela

    Verified customer

    Submitted Jun 23 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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