Product nameAnti-Star-PAP antibody
See all Star-PAP primary antibodies
DescriptionRabbit polyclonal to Star-PAP
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Cat, Dog
- 293T cell lysate.
Previously labelled as TUT1.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 2% Sucrose
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86161 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 94 kDa (predicted molecular weight: 94 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionPoly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the DNA polymerase type-B-like family.
Contains 1 C2H2-type zinc finger.
Contains 1 PAP-associated domain.
Contains 1 RRM (RNA recognition motif) domain.
modificationsPhosphorylated by CK1 in the proline-rich (Pro-rich) region. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nucleolus. Nucleus speckle.
- Information by UniProt
- alpha regulated poly(A) polymerase antibody
- nuclear speckle targeted phosphatidylinositol 4 phosphate 5 kinase type I antibody
- nuclear speckle targeted PIPK1A regulated poly(A) polymerase antibody
Anti-Star-PAP antibody (ab86161) at 1 µg/ml (5% skim milk / PBS buffer) + 293T cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 94 kDa
Observed band size: 94 kDa
Additional bands at: >168 kDa, 45 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 6-18%
ab86161 has not yet been referenced specifically in any publications.