Overview

  • Product name

  • Description

    Rabbit polyclonal to StARD13
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence: NPVMLDAPLV SSSLPQPPRD VLNHPFHPKN EKPTRARAKS FLKRMETLRG KGAHGRHKGS GRTGGLVISG PMLQQEPESF KAMQCIQIPN GDLQNSPPPA CRKGLPC, corresponding to amino acids of Human StARD13.

  • Positive control

    • Human pancreas tissue; U251 MG and Human liver tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab126489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500. Predicted molecular weight: 125 kDa.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    GTPase-activating protein for RhoA, and perhaps for Cdc42. May be involved in regulation of cytoskeletal reorganization, cell proliferation and cell motility. Acts a tumor suppressor in hepatocellular carcinoma cells.
  • Tissue specificity

    Ubiquitously expressed. Underexpressed in hepatocellular carcinoma cells and some breast cancer cell lines.
  • Sequence similarities

    Contains 1 Rho-GAP domain.
    Contains 1 SAM (sterile alpha motif) domain.
    Contains 1 START domain.
  • Cellular localization

    Cytoplasm. Membrane. Mitochondrion membrane. Lipid droplet.
  • Information by UniProt
  • Database links

  • Alternative names

    • 46H23.2 antibody
    • ARHGAP37 antibody
    • Deleted in liver cancer 2 protein antibody
    • Deleted in liver cancer protein 2 antibody
    • DLC-2 antibody
    • DLC2 antibody
    • GT650 antibody
    • Rho GTPase activating protein on chromosome 13q12 antibody
    • Rho GTPase-activating protein antibody
    • STA13 antibody
    • STA13_HUMAN antibody
    • StAR related lipid transfer (START) domain containing 13 antibody
    • StAR-related lipid transfer protein 13 antibody
    • StARD13 antibody
    • START domain-containing protein 13 antibody
    see all

Images

  • ab126489 at 1/200 dilution staining StARD13 in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
  • All lanes : Anti-StARD13 antibody (ab126489) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate

    Developed using the ECL technique.

    Predicted band size: 125 kDa

References

This product has been referenced in:

  • Yang Z  et al. DLC2 operates as a tumor suppressor gene in breast cancer via the RhoGTPase pathway. Oncol Lett 17:2107-2116 (2019). Read more (PubMed: 30719106) »
  • Gao F  et al. STARD13 is positively correlated with good prognosis and enhances 5-FU sensitivity via suppressing cancer stemness in hepatocellular carcinoma cells. Onco Targets Ther 11:5371-5381 (2018). Read more (PubMed: 30214243) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for providing details of the sample types.

Several isoforms (5) have been reported and it is very likely that the double band in certain lysates (HEK293T, HeLa, U251MG) represent some of the variants of StARD13. You and your customers may wish to take a look at this site for further information:

http://www.uniprot.org/uniprot/Q9Y3M8

If you need any further assistance in the future, please do not hesitate to contact me.

Read More

Question

Dear technical team,

Our customer tested WB with ab126489 and it showed 2 bands.
I have checked that there’re 5 isoforms but please check the attached file and explain about those 2 bands.


1) Abcam product code

Ab126489

2) Abcam order reference number or product batch number

GR80689, ON#1063828

2-1) storage temperature of antibody

-20℃

3) Description of the problem

It detected the two bands near the 130kDa

4) Sample preparation:

Type of sample and species :

whole cell lysates and Human stomach and breast cancer cell

Lysis buffer : RIPA or NP-40

Protease inhibitors: PMSF, Aprotinin , Leupeptin, Pepstatin

Phosphatase inhibitors Sodium orthovanadate

Reducing agent beta-mercaptoethanol

Boiling for ≥5 min? yes ,

Protein loaded ug/lane or cells/lane 30ug

Positive control U251MG

Negative control RT4



5) Percentage of gel 8% SDS PAGE gel

Type of membrane PDVF membrane

Protein transfer verified using Ponceou S (5min staining)

Blocking agent and concentration 5% skim milk in 1x TBST buffer

Blocking time 2hours

Blocking temperature Room Temperature



6) Primary antibody (If more than one was used, describe in “additional notes”) :

Concentration or dilution 1:500

Diluent buffer 1% BSA

Incubation time over night(about 16˜18hr)

Incubation temperature: 4℃



7) Secondary antibody: goat anti-rabbit IgG-HRP: sc-2004

Species: goat

Isotype: IgG

Reacts against:

Concentration or dilution 1:2000

Diluent buffer 5% skim milk

Incubation time 2hr

Incubation temperature: Room Temperature

Fluorochrome or enzyme conjugate: HRP



8) Washing after primary and secondary antibodies:

Buffer 1x TBST

Number of washes 4 times



9)Detection method ECL

10) How many times have you run this staining? 5 times

-Do you obtain the same results every time? yes

-Have you run a "No Primary" control? Yes (negative and positive control)

-What steps have you altered to try and optimize the use of this antibody? Change of concentration (1:250)



Document attachment: Attaching images of your blot is strongly recommended and can greatly speed up our investigation of your problem.



à 130kDa
Best regards

Read More
Answer

Thank you for your enquiry regarding ab126489 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

Though you have kindly provided some details, it would be much appreciated if I could get some more information which would help me identify the source of the problem.

Q1: Could you please explain what lanes A, B and C represent on the Western blot image? It seems that the Lane A has only one single band.

Q2: What was applied as positive control (species, origin of cells) and negative control (species, origin of cells)?

Thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.

Read More

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