Anti-STARD3NL antibody (ab121592)
Key features and details
- Rabbit polyclonal to STARD3NL
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-STARD3NL antibody
See all STARD3NL primary antibodies -
Description
Rabbit polyclonal to STARD3NL -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: MNHLPEDMEN ALTGSQSSHA SLRNIHSINP TQLMARIESY EGREKKGISD VRR, corresponding to N terminal amino acids 1-53 of Human STARD3NL.
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Positive control
- Human duodenum tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab121592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.04 - 0.4 µg/ml. | |
| ICC/IF | Use a concentration of 0.25 - 2 µg/ml. | |
| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Contains 1 MENTAL domain. -
Cellular localization
Late endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 83930 Human
- Omim: 611759 Human
- SwissProt: O95772 Human
- Unigene: 728820 Human
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Alternative names
- MENTO_HUMAN antibody
- MLN64 N-terminal domain homolog antibody
- STARD3 N-terminal-like protein antibody
- Stard3nl antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-STARD3NL antibody (ab121592)Immunofluorescent staining of Human cell line U-2 OS shows positivity in vesicles. Recommended concentration of ab121592 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STARD3NL antibody (ab121592)ab121592, at a 1/75 dilution, staining STARD3NL in glandular cells of paraffin-embedded Human duodenum tissue by Immunohistochemistry. -
Western blot - Anti-STARD3NL antibody (ab121592)Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
Protocols
Datasheets and documents
References (0)
ab121592 has not yet been referenced specifically in any publications.
