- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-STARD3NL antibody
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DescriptionRabbit polyclonal to STARD3NL
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
antigen sequence: MNHLPEDMEN ALTGSQSSHA SLRNIHSINP TQLMARIESY EGREKKGISD VRR, corresponding to N terminal amino acids 1-53 of Human STARD3NL.
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Positive control
- Human duodenum tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab121592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. | |
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
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| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similaritiesContains 1 MENTAL domain.
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Cellular localizationLate endosome membrane.
- Information by UniProt
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Database links
- Entrez Gene: 83930 Human
- Omim: 611759 Human
- SwissProt: O95772 Human
- Unigene: 728820 Human
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Alternative names
- MENTO_HUMAN antibody
- MLN64 N-terminal domain homolog antibody
- STARD3 N-terminal-like protein antibody
- Stard3nl antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-STARD3NL antibody (ab121592)Immunofluorescent staining of Human cell line U-2 OS shows positivity in vesicles. Recommended concentration of ab121592 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STARD3NL antibody (ab121592)ab121592, at a 1/75 dilution, staining STARD3NL in glandular cells of paraffin-embedded Human duodenum tissue by Immunohistochemistry. -
Western blot - Anti-STARD3NL antibody (ab121592)Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
Protocols
References
ab121592 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"