Recombinant Anti-STARD5 antibody [EPR13545(B)] (ab178688)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13545(B)] to STARD5
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-STARD5 antibody [EPR13545(B)]
See all STARD5 primary antibodies -
Description
Rabbit monoclonal [EPR13545(B)] to STARD5 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human STARD5 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9NSY2 -
Positive control
- HepG2, 293T, THP-1, human fetal liver, human fetal kidney and HL-60 lysates. Permeabilized 293T cells. Immunoprecipitation pellet from THP-1 cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13545(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178688 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
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IP |
1/10 - 1/100.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 24 kDa. |
IP
1/10 - 1/100. |
Target
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Relevance
STARD5, or START domain containing 5, belongs to a family containing a 200- to 210-amino acid STAR-related lipid transfer (START) domain. STARD5 may be involved in the intracellular transport of sterols or other lipids and may bind cholesterol or other sterols. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 80765 Human
- Entrez Gene: 170460 Mouse
- Entrez Gene: 502348 Rat
- Omim: 607050 Human
- SwissProt: Q9NSY2 Human
- SwissProt: Q9EPQ7 Mouse
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Alternative names
- MGC10327 antibody
- StAR related lipid transfer protein 5 antibody
- StAR-related lipid transfer (START) domain containing 5 antibody
see all
Images
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All lanes : Anti-STARD5 antibody [EPR13545(B)] (ab178688) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 3 : THP-1 lysate
Lane 4 : Human fetal liver tissue lysate
Lane 5 : Human fetal kidney tissue lysate
Lane 6 : HL-60 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 24 kDa -
Intracellular flow cytometric analysis of permeabilized 293T cells using ab178688 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
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Western blot analysis on immunoprecipitation pellet from (Lane 1) THP-1 cell lysate or (Lane 2) 1X PBS (negative control) using ab178688 at a 1/10 dilution for IP and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab178688 has not yet been referenced specifically in any publications.