Anti-Stargazin antibody [N245/36] (ab167445)

Overview

  • Product name
    Anti-Stargazin antibody [N245/36]
  • Description
    Mouse monoclonal [N245/36] to Stargazin
  • Host species
    Mouse
  • Specificity
    ab167445 does not cross-react with Stargazin 3.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Fusion protein, corresponding to C terminal amino acids 203-323 of Rat Stargazin (NP_445803.1).

  • Positive control
    • WB: Rat brain tissue lysate. ICC/IF: SK-N-BE cells.
  • General notes

    The clone number has been updated from S245-36 to N245/36, both clone numbers name the same antibody clone.

     

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    N245/36
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab167445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 36 kDa.
ICC/IF 1/100.

Target

  • Function
    Regulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization. Does not show subunit-specific AMPA receptor regulation and regulates all AMPAR subunits. Thought to stabilize the calcium channel in an inactivated (closed) state.
  • Tissue specificity
    Brain.
  • Sequence similarities
    Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily.
  • Post-translational
    modifications
    Phosphorylation of Thr-321 impairs interaction with DLG1 and DLG4.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AW060990 antibody
    • B230105C07Rik antibody
    • B930041E13Rik antibody
    • Cacng2 antibody
    • Calcium channel voltage dependent gamma subunit 2 antibody
    • CaV gamma 2 antibody
    • CCG2_HUMAN antibody
    • Ipr328 antibody
    • MGC123981 antibody
    • MGC138502 antibody
    • MGC138504 antibody
    • Neuronal voltage gated calcium channel gamma 2 subunit antibody
    • Neuronal voltage-gated calcium channel gamma-2 subunit antibody
    • Stargazer antibody
    • stg antibody
    • TARP gamma-2 antibody
    • Transmembrane AMPAR regulatory protein gamma-2 antibody
    • Voltage dependent calcium channel gamma 2 subunit antibody
    • Voltage-dependent calcium channel gamma-2 subunit antibody
    • Wag antibody
    • Waggler antibody
    see all

Images

  • 4% formaldehyde-fixed SK-N-BE cells labeling Stargazin using ab167445 at 1/100 dilution in ICC/IF. Cells were fixed for 15 minutes at room temperature and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 (green) at 1/100 dilution incubated for 1 hour at room temperature. Counterstained with Phalloidin Texas Red F-actin stain. Nuclei were stained with DAPI (blue).

  • Lane 1 : Anti-Stargazin antibody [N245/36] (ab167445) at 1/100 dilution
    Lane 2 : Anti-Stargazin antibody [N245/36] (ab167445) at 1/250 dilution
    Lane 3 : Anti-Stargazin antibody [N245/36] (ab167445) at 1/500 dilution
    Lane 4 : Anti-Stargazin antibody [N245/36] (ab167445) at 1/1000 dilution

    All lanes : Rat brain lysate

    Predicted band size: 36 kDa

References

ab167445 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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