Key features and details
- Mouse monoclonal [N245/36] to Stargazin
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG2a
Product nameAnti-Stargazin antibody [N245/36]
See all Stargazin primary antibodies
DescriptionMouse monoclonal [N245/36] to Stargazin
Specificityab167445 does not cross-react with Stargazin 3.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat
- WB: Rat brain tissue lysate. ICC/IF: SK-N-BE cells.
The clone number has been updated from S245-36 to N245/36, both clone numbers name the same antibody clone.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab167445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 36 kDa.|
FunctionRegulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization. Does not show subunit-specific AMPA receptor regulation and regulates all AMPAR subunits. Thought to stabilize the calcium channel in an inactivated (closed) state.
Sequence similaritiesBelongs to the PMP-22/EMP/MP20 family. CACNG subfamily.
modificationsPhosphorylation of Thr-321 impairs interaction with DLG1 and DLG4.
- Information by UniProt
- AW060990 antibody
- B230105C07Rik antibody
- B930041E13Rik antibody
4% formaldehyde-fixed SK-N-BE cells labeling Stargazin using ab167445 at 1/100 dilution in ICC/IF. Cells were fixed for 15 minutes at room temperature and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 (green) at 1/100 dilution incubated for 1 hour at room temperature. Counterstained with Phalloidin Texas Red F-actin stain. Nuclei were stained with DAPI (blue).
Lane 1 : Anti-Stargazin antibody [N245/36] (ab167445) at 1/100 dilution
Lane 2 : Anti-Stargazin antibody [N245/36] (ab167445) at 1/250 dilution
Lane 3 : Anti-Stargazin antibody [N245/36] (ab167445) at 1/500 dilution
Lane 4 : Anti-Stargazin antibody [N245/36] (ab167445) at 1/1000 dilution
All lanes : Rat brain lysate
Predicted band size: 36 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab167445 has not yet been referenced specifically in any publications.