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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper Leucine Zipper
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RecombinantRabMAb

Recombinant Anti-STAT1 (phospho Y701) antibody [EPR3147] (ab109457)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (1)

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Dot Blot - Anti-STAT1 (phospho Y701) antibody [EPR3147] (ab109457)
  • Western blot - Anti-STAT1 (phospho Y701) antibody [EPR3147] (ab109457)
  • Western blot - Anti-STAT1 (phospho Y701) antibody [EPR3147] (ab109457)
  • Anti-STAT1 (phospho Y701) antibody [EPR3147] (ab109457)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3147] to STAT1 (phospho Y701)
  • Suitable for: WB, Dot blot
  • Reacts with: Human

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Overview

  • Product name

    Anti-STAT1 (phospho Y701) antibody [EPR3147]
    See all STAT1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3147] to STAT1 (phospho Y701)
  • Host species

    Rabbit
  • Specificity

    This antibody only detects STAT1 when phosphorylated at Tyrosine 701.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431 cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR3147
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • Leucine Zipper
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • STATs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • STATs
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • STAT family

Associated products

  • Alternative Versions

    • Anti-STAT1 (phospho Y701) antibody [EPR3147] - BSA and Azide free (ab247870)
  • Assay kits

    • STAT (STAT1alpha, STAT3, STAT5A, STAT5B) Transcription Factor Assay Kit (Colorimetric) (ab207228)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human STAT1 protein (ab82610)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 87 kDa.
Dot blot
1/1000.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 87 kDa.
Dot blot
1/1000.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.
    • Involvement in disease

      Note=STAT1 deficiency results in impaired immune response leading to severe mycobacterial and viral diseases. In the case of complete deficiency, patients can die of viral disease.
      Defects in STAT1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
    • Sequence similarities

      Belongs to the transcription factor STAT family.
      Contains 1 SH2 domain.
    • Post-translational
      modifications

      Phosphorylated on tyrosine and serine residues in response to IFN-alpha, IFN-gamma, PDGF and EGF. Phosphorylation on Tyr-701 (lacking in beta form) by JAK promotes dimerization and subsequent translocation to the nucleus. Phosphorylation on Ser-727 by several kinases including MAPK14, ERK1/2 and CAMKII on IFN-gamma stimulation, regulates STAT1 transcriptional activity. Phosphorylation on Ser-727 promotes sumoylation though increasing interaction with PIAS. Phosphorylation on Ser-727 by PKCdelta induces apoptosis in response to DNA-damaging agents.
      Sumoylated by SUMO1, SUMO2 and SUMO3. Sumoylation is enhanced by IFN-gamma-induced phosphorylation on Ser-727, and by interaction with PIAS proteins. Enhances the transactivation activity.
      ISGylated.
    • Cellular localization

      Cytoplasm. Nucleus. Translocated into the nucleus in response to IFN-gamma-induced tyrosine phosphorylation and dimerization.
    • Target information above from: UniProt accession P42224 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6772 Human
      • Omim: 600555 Human
      • SwissProt: P42224 Human
      • Unigene: 642990 Human
      • Unigene: 743244 Human
      • Alternative names

        • Signal transducer and activator of transcription 1 91kD antibody
        • CANDF7 antibody
        • DKFZp686B04100 antibody
        • IMD31A antibody
        • IMD31B antibody
        • IMD31C antibody
        • ISGF 3 antibody
        • ISGF-3 antibody
        • OTTHUMP00000163552 antibody
        • OTTHUMP00000165046 antibody
        • OTTHUMP00000165047 antibody
        • OTTHUMP00000205845 antibody
        • Signal transducer and activator of transcription 1 91kDa antibody
        • Signal transducer and activator of transcription 1 antibody
        • Signal transducer and activator of transcription 1, 91kD antibody
        • Signal transducer and activator of transcription 1-alpha/beta antibody
        • STAT 1 antibody
        • Stat1 antibody
        • STAT1_HUMAN antibody
        • STAT91 antibody
        • Transcription factor ISGF 3 components p91 p84 antibody
        • Transcription factor ISGF-3 components p91/p84 antibody
        • Transcription factor ISGF3 components p91/p84 antibody
        • XStat1 antibody
        see all

      Images

      • Dot Blot - Anti-STAT1 (phospho Y701) antibody [EPR3147] (ab109457)
        Dot Blot - Anti-STAT1 (phospho Y701) antibody [EPR3147] (ab109457)

        Primary antibody dilution: 1/1000

        Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated

        Secondary antibody dilution: 1/2500

        Blocking & dilution buffer: 5% NFDM/TBST

        Lane 1 sample: STAT1 (pY701) phospho peptide

        Lane 2 sample: STAT1 non-phospho peptide

        Exposure time: 3 minutes

      • Western blot - Anti-STAT1 (phospho Y701) antibody [EPR3147] (ab109457)
        Western blot - Anti-STAT1 (phospho Y701) antibody [EPR3147] (ab109457)
        All lanes : Anti-STAT1 (phospho Y701) antibody [EPR3147] (ab109457)

        Lane 1 : A431 (human epidermoid carcinoma) treated with Epidermal Growth Factor (EGF) whole cell lysates
        Lane 2 : Untreated A431 (human epidermoid carcinoma) whole cell lysates

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

        Predicted band size: 87 kDa
        Observed band size: 91 kDa why is the actual band size different from the predicted?


        Exposure time: 3 minutes


        Blocking and dilution buffer: 5% NFDM /TBST

      • Western blot - Anti-STAT1 (phospho Y701) antibody [EPR3147] (ab109457)
        Western blot - Anti-STAT1 (phospho Y701) antibody [EPR3147] (ab109457)
        All lanes : Anti-STAT1 (phospho Y701) antibody [EPR3147] (ab109457) at 1/1000 dilution

        Lane 1 : A431 cell lysates untreated with Epidermal Growth Factor (EGF).
        Lane 2 : A431 cell lysates treated with Epidermal Growth Factor (EGF).

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 87 kDa



        The loading control is Anti-stat1 antibody, ab92506. 1/1000 dilution.

      • Anti-STAT1 (phospho Y701) antibody [EPR3147] (ab109457)
        Anti-STAT1 (phospho Y701) antibody [EPR3147] (ab109457)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab109457? Please let us know so that we can cite the reference in this datasheet.

      ab109457 has been referenced in 1 publication.

      • Zhang J  et al. miR-26a Inhibits Feline Herpesvirus 1 Replication by Targeting SOCS5 and Promoting Type I Interferon Signaling. Viruses 12:N/A (2019). PubMed: 31861450

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-2 of 2 Abreviews or Q&A

      Western blot abreview for Anti-STAT1 (phospho Y701) antibody [EPR3147]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Hela)
      Gel Running Conditions
      Reduced Denaturing (4-12% BisTris Gel)
      Loading amount
      10 µg
      Treatment
      IFNalpha
      Specification
      Hela
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More

      Abcam user community

      Verified customer

      Submitted Apr 03 2019

      Question

      I am interested in measuring STAT1-alpha in PBMC. I have found Anti-STAT1 alpha (phospho Y701) antibody [EPR3147] (ab109457) in the abcam website but am not sure whether it targets both STAT-1 alpha and beta. This is because STAT1-alpha is 91 kDa and in the abcam antibody sheet it is mentioned that it is 87 KDa. Can you please clarify this.

      Read More

      Abcam community

      Verified customer

      Asked on Feb 22 2012

      Answer

      Thank you for contacting us.

      The predicted band size that we have on our website is based upon the information available on the Uniprot/Swiss Prot page:http://www.uniprot.org/uniprot/P42224. This is linked on the datasheet as well and lists the Alpha isoform at 89kDa and Beta isoform at 83kDa.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Feb 22 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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