Product nameAnti-STAT1 (phospho Y701) antibody [M135]
See all STAT1 primary antibodies
DescriptionMouse monoclonal [M135] to STAT1 (phospho Y701)
SpecificityThe antibody detects 84 and 91 kDa stat1 variants on SDS-PAGE immunoblots of human A431 treated with EGF, as well as Jurkat and A431 cells treated with pervanadate. The antibody does not detect these variants in control cells.
Tested applicationsSuitable for: ICC, WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide corresponding to Human STAT1 (phospho Y701) conjugated to Keyhole Limpet Haemocyanin (KLH).
- human SKOV-3 and MDA-MB-468 cells, ab7909, Jurkat cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 50% PBS, 50% Glycerol, 0.1% BSA
Concentration information loading...
PurityProtein A purified
Purification notesMouse monoclonal antibody purified with protein A chromatography.
Our Abpromise guarantee covers the use of ab29045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|WB||1/1000. Predicted molecular weight: 87 kDa.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 21677781|
FunctionSignal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.
Involvement in diseaseNote=STAT1 deficiency results in impaired immune response leading to severe mycobacterial and viral diseases. In the case of complete deficiency, patients can die of viral disease.
Defects in STAT1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
Sequence similaritiesBelongs to the transcription factor STAT family.
Contains 1 SH2 domain.
modificationsPhosphorylated on tyrosine and serine residues in response to IFN-alpha, IFN-gamma, PDGF and EGF. Phosphorylation on Tyr-701 (lacking in beta form) by JAK promotes dimerization and subsequent translocation to the nucleus. Phosphorylation on Ser-727 by several kinases including MAPK14, ERK1/2 and CAMKII on IFN-gamma stimulation, regulates STAT1 transcriptional activity. Phosphorylation on Ser-727 promotes sumoylation though increasing interaction with PIAS. Phosphorylation on Ser-727 by PKCdelta induces apoptosis in response to DNA-damaging agents.
Sumoylated by SUMO1, SUMO2 and SUMO3. Sumoylation is enhanced by IFN-gamma-induced phosphorylation on Ser-727, and by interaction with PIAS proteins. Enhances the transactivation activity.
Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus in response to IFN-gamma-induced tyrosine phosphorylation and dimerization.
- Information by UniProt
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Lanes 1-2 & 4 : Anti-STAT1 antibody (ab2415)
Lane 3 : Anti-STAT1 (phospho Y701) antibody [M135] (ab29045)
Lanes 5-6 : Anti-STAT3 antibody (ab5073)
Lanes 7-8 : Anti-STAT5 antibody (ab68465)
Lanes 9-10 : anti-Stat5 (Tyr-694)
All lanes :
A431 whole cell lysate (ab7909)
Predicted band size: 87 kDa
Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8, & 10).
ab29045 staining STAT1 (phospho Y701) in human SKOV-3 and MDA-MB-468 cells by Immunocytochemistry/ Immunofluorescence. The cells were EDTA/trypsin treated then fixed in 10% formaldehyde and blocked using 2% BSA for 1 hour. Samples were then incubated with primary antibody at 1/100 for 18 hours at 4°C. The secondary antibody used was an anti-mouse IgG conjugated to HRP used at a 1/1000 dilution.
Lanes 1-2 : Anti-Stat1
Lanes 3-4 : Anti-STAT1 (phospho Y701) antibody [M135] (ab29045) at 1/1000 dilution
Lanes 1 & 3 : Jurkat cells
Lanes 2 & 4 : Jurkat cells treated with 1mM pervanadate
Predicted band size: 87 kDa
Observed band size: 84,91 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Zhang S et al. Swiprosin-1 deficiency impairs macrophage immune response of septic mice. JCI Insight 3:N/A (2018). Read more (PubMed: 29415882) »
- Boulay AC et al. Connexin 43 Controls the Astrocyte Immunoregulatory Phenotype. Brain Sci 8:N/A (2018). Read more (PubMed: 29565275) »