Anti-STAT1beta antibody - C-terminal (ab177412)

Overview

  • Product name

    Anti-STAT1beta antibody - C-terminal
  • Description

    Rabbit polyclonal to STAT1beta - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human STAT1beta (C terminal). The exact sequence is proprietary. ab177412 was raised against a 15 amino acid peptide near the carboxy terminus
    Database link: P42224-2

  • Positive control

    • Human small intestine tissue lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This antibody was purified by affinity chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab177412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 83 kDa.

Target

  • Relevance

    STAT1 is a member of the signal transducers and activators of transcription (STAT) family of proteins that carry out a dual function: signal transduction and activation of transcription. STAT1 transcription factor is specific for the IFN pathway and plays a central role in mediating many, if not all, IFN-dependent biological responses. Presence of STAT1 leads to an efficient antiviral response when cells were infected with virus suggesting that a STAT-dependent pathway is activated following virus infection by endogenously produced IFN. Virus-induced STAT protein translocation from the cytoplasmic compartment can be detected within 3 h of infection.
  • Cellular localization

    Cytoplasmic and Nuclear
  • Database links

  • Alternative names

    • CANDF7 antibody
    • ISGF-3 antibody
    • p84 antibody
    • signal transducer and activator of transcription 1, 84kDa antibody
    • signal transducer and activator of transcription 1-alpha/beta antibody
    • signal transducer and activator of transcription-1 antibody
    • STAT1 p84 antibody
    • transcription factor ISGF-3 components p91/p84 antibody
    see all

Images

  • Lane 1 : Anti-STAT1beta antibody - C-terminal (ab177412) at 1 µg/ml
    Lane 2 : Anti-STAT1beta antibody - C-terminal (ab177412) at 2 µg/ml

    All lanes : Human small intestine tissue lysate

    Predicted band size: 83 kDa

References

ab177412 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (ARPE-19 lysates)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Gel)
Loading amount
10 µg
Treatment
125 ug/ml CSE for 24 hrs
Specification
ARPE-19 lysates
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 13 2019

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