Recombinant Anti-STAT2 antibody [22/Stat2] (ab280900)
![Western blot - Anti-STAT2 antibody [22/Stat2] (ab280900)](https://www.abcam.com/ps/products/280/ab280900/Images/ab280900-1-anti-stat2-antibody-22stat2-western-blot-wildtype-hela.jpg "Western blot - Anti-STAT2 antibody [22/Stat2] (ab280900)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [22/Stat2] to STAT2
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-STAT2 antibody [22/Stat2]
See all STAT2 primary antibodies -
Description
Mouse monoclonal [22/Stat2] to STAT2 -
Host species
Mouse -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: ICC -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild type HeLa whole cell lysate. Ramos, K-562 and THP1 whole cell lysate. IP: Ramos whole cell lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
22/Stat2 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab280900 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 97 kDa.
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| IP |
1/30.
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| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 97 kDa. |
|
IP
1/30. |
Target
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Function
Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. -
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
Post-translational
modificationsTyrosine phosphorylated in response to IFN-alpha. -
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta. - Information by UniProt
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Database links
- Entrez Gene: 6773 Human
- Omim: 600556 Human
- SwissProt: P52630 Human
- Unigene: 530595 Human
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Alternative names
- Homo sapiens interferon alpha induced transcriptional activator antibody
- interferon alpha induced transcriptional activator antibody
- ISGF 3 antibody
see all
Images
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Western blot - Anti-STAT2 antibody [22/Stat2] (ab280900)All lanes : Anti-STAT2 antibody [22/Stat2] (ab280900) at 1/1000 dilution
Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate
Lane 2 : STAT2 knockout HeLa whole cell lysate
Lane 3 : Ramos (Human Burkitt’s lymphoma B lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (IRDye® 800CW) (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) (ab216777) at 1/10000 dilution
Predicted band size: 97 kDa
Observed band size: 97,113 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS.
Lanes 1 - 3: Merged signal (red and green). Green - ab280900 observed at 97, 113 kDa. Red - loading control ab181602 (Rabbit monoclonal [EPR16891] to GAPDH) observed at 36 kDa.
Lanes 1-2: ab280900 Anti-Stat2 antibody was shown to react with STAT2 in HeLa cells in Western blot. Loss of signal was observed when STAT2 knockout sample was used. Wild-type and STAT2 knockout samples were subjected to SDS-PAGE.
ab280900 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated at 4°C overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680CW) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800RD) preadsorbed (ab216772) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-STAT2 antibody [22/Stat2] (ab280900)](https://www.abcam.com/ps/products/280/ab280900/Images/ab280900-3-anti-stat2-antibody-22stat2-immunoprecipitation-ramos.jpg)
Immunoprecipitation - Anti-STAT2 antibody [22/Stat2] (ab280900)Stat2 was immunoprecipitated from 0.35 mg Ramos (Human Burkitt's lymphoma B lymphocyte), whole cell lysate 10 µg with ab280900 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab280900 at 1/1000 dilution. Mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution.
Lane 1: Ramos whole cell lysate 10 µg
Lane 2: ab280900 IP in Ramos whole cell lysate
Lane 3: Mouse monoclonal IgG2a (ab18413) instead of ab280900 in Ramos whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Western blot - Anti-STAT2 antibody [22/Stat2] (ab280900)](https://www.abcam.com/ps/products/280/ab280900/Images/ab280900-2-anti-stat2-antibody-22stat2-western-blot-k562.jpg)
Western blot - Anti-STAT2 antibody [22/Stat2] (ab280900)All lanes : Anti-STAT2 antibody [22/Stat2] (ab280900) at 1/1000 dilution
Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 2 : Ramos (Human Burkitt’s lymphoma B lymphocyte), whole cell lysate
Lane 3 : THP1 (human monocytic leukemia monocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 97 kDa
Observed band size: 97,113 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 92 seconds
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![Anti-STAT2 antibody [22/Stat2] (ab280900)](https://www.abcam.com/ps/products/280/ab280900/Images/ab280900-4-Benefits-of-Recombinant-antibodies.jpg)
Anti-STAT2 antibody [22/Stat2] (ab280900)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab280900 has not yet been referenced specifically in any publications.
