Anti-STAT2 antibody (ab124283)
Key features and details
- Rabbit polyclonal to STAT2
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-STAT2 antibody
See all STAT2 primary antibodies -
Description
Rabbit polyclonal to STAT2 -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human STAT2 aa 150-250.
Database link: P52630 -
Positive control
- IP: Jurkat whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab124283 was affinity purified using an epitope specific to STAT2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. -
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
Post-translational
modificationsTyrosine phosphorylated in response to IFN-alpha. -
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta. - Information by UniProt
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Database links
- Entrez Gene: 6773 Human
- Omim: 600556 Human
- SwissProt: P52630 Human
- Unigene: 530595 Human
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Alternative names
- Homo sapiens interferon alpha induced transcriptional activator antibody
- interferon alpha induced transcriptional activator antibody
- ISGF 3 antibody
see all
Images
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Detection of STAT2 by Western Blot of Immunprecipitate.
anti-STAT2 antibody at 1µg/ml staining STAT2 in Jurkat whole cell lysate immunoprecipitated using ab123283 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab124283 has been referenced in 2 publications.
- Hu Y et al. LncRNA DLX6-AS1 Promotes the Progression of Neuroblastoma by Activating STAT2 via Targeting miR-506-3p. Cancer Manag Res 12:7451-7463 (2020). PubMed: 32904436
- Iwata S et al. The Transcription Factor T-bet Limits Amplification of Type I IFN Transcriptome and Circuitry in T Helper 1 Cells. Immunity 46:983-991.e4 (2017). PubMed: 28623086