Key features and details
- Rabbit polyclonal to STAT2
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-STAT2 antibody
See all STAT2 primary antibodies
DescriptionRabbit polyclonal to STAT2
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 175-225 of Human STAT2 (NP_005410.1).
- Jurkat whole cell lysate.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab124283 was affinity purified using an epitope specific to STAT2 immobilized on solid support.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
Use at 2-10 µg/mg of lysate.
FunctionSignal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
Sequence similaritiesBelongs to the transcription factor STAT family.
Contains 1 SH2 domain.
modificationsTyrosine phosphorylated in response to IFN-alpha.
Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.
- Information by UniProt
- Homo sapiens interferon alpha induced transcriptional activator antibody
- interferon alpha induced transcriptional activator antibody
- ISGF 3 antibody
Detection of STAT2 by Western Blot of Immunprecipitate.
anti-STAT2 antibody at 1µg/ml staining STAT2 in Jurkat whole cell lysate immunoprecipitated using ab123283 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
ab124283 has been referenced in 1 publication.
- Iwata S et al. The Transcription Factor T-bet Limits Amplification of Type I IFN Transcriptome and Circuitry in T Helper 1 Cells. Immunity 46:983-991.e4 (2017). PubMed: 28623086