Key features and details
- Rabbit polyclonal to STAT2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-STAT2 antibody
See all STAT2 primary antibodies
DescriptionRabbit polyclonal to STAT2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat
Recombinant fragment corresponding to Rat STAT2 aa 587-838.
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEFRRLLKKMVSGTFLL RFSETSEGGITCSWVEHQDDDKVLIYSVQPYTKEVLQSLPLTEIIRHYQV LAEENAPENPLRFLYPRVPRDEAFGCYYQEKVNLEERRKYLKHRLIVISN RQADELQQPLELKQDPESLELDAELMSVMELARDLELQSMLQVGLDLGVE PKPDPTLSPAPQILLEPDPARALNQLLPEPDLPQDLQQLNTGEMEIFRNN INIDDVMPYGDPVLAGQSIIEEAYRSCPSHFDVDGPLI
Database link: Q5XI26
- WB: Recombinant rat STAT2 protein; Rat serum; Mouse lung lysate. IHC-P: Rat stomach, adrenal gland, liver, spinal cord, skin and lung tissues.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab231184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 97 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionSignal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
Sequence similaritiesBelongs to the transcription factor STAT family.
Contains 1 SH2 domain.
modificationsTyrosine phosphorylated in response to IFN-alpha.
Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.
- Information by UniProt
- Homo sapiens interferon alpha induced transcriptional activator antibody
- interferon alpha induced transcriptional activator antibody
- ISGF 3 antibody
Anti-STAT2 antibody (ab231184) at 3 µg + Recombinant rat STAT2 protein
Predicted band size: 97 kDa
All lanes : Anti-STAT2 antibody (ab231184) at 3 µg/ml
Lane 1 : Rat serum
Lane 2 : Mouse lung lysate
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 97 kDa
Formalin-fixed, paraffin-embedded rat stomach tissue stained for STAT2 with ab231184 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded rat adrenal gland tissue stained for STAT2 with ab231184 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded rat liver tissue stained for STAT2 with ab231184 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded rat spinal cord tissue stained for STAT2 with ab231184 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded rat skin tissue stained for STAT2 with ab231184 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded rat lung tissue stained for STAT2 with ab231184 at 20 μg/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231184 has been referenced in 3 publications.
- Guan G et al. Down-regulation of IFIT3 protects liver from ischemia-reperfusion injury. Int Immunopharmacol 60:170-178 (2018). WB ; Mouse . PubMed: 29734133
- Zhang J et al. Identification of NPAC as a novel biomarker and regulator for hepatocellular carcinoma. J Cell Biochem N/A:N/A (2018). PubMed: 30474880
- Xia L et al. Detection of host pathways universally inhibited after Plasmodium yoelii infection for immune intervention. Sci Rep 8:15280 (2018). PubMed: 30327482