Anti-STAT2 antibody (ab233177)
Key features and details
- Rabbit polyclonal to STAT2
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-STAT2 antibody
See all STAT2 primary antibodies -
Description
Rabbit polyclonal to STAT2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human STAT2 aa 600-850. Two N-terminal tags. (Expressed in E.coli).
Database link: P52630 -
Positive control
- IHC-P: Human stomach, kidney, stomach cancer and liver tissues. WB: K562 and HeLa cell lysates; Pig liver lysate; Recombinant human STAT2 protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab233177 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 97 kDa.
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IHC-P | (1) |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 97 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. -
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
Post-translational
modificationsTyrosine phosphorylated in response to IFN-alpha. -
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta. - Information by UniProt
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Database links
- Entrez Gene: 6773 Human
- Omim: 600556 Human
- SwissProt: P52630 Human
- Unigene: 530595 Human
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Alternative names
- Homo sapiens interferon alpha induced transcriptional activator antibody
- interferon alpha induced transcriptional activator antibody
- ISGF 3 antibody
see all
Images
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Paraffin-embedded human stomach tissue stained for STAT2 using ab233177 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human kidney tissue stained for STAT2 using ab233177 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human stomach cancer tissue stained for STAT2 using ab233177 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Anti-STAT2 antibody (ab233177) at 2 µg/ml + Recombinant human STAT2 protein
Predicted band size: 97 kDa -
Anti-STAT2 antibody (ab233177) at 2 µg/ml + K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Predicted band size: 97 kDa -
Anti-STAT2 antibody (ab233177) at 2 µg/ml + Pig liver tissue lysate
Predicted band size: 97 kDa -
Anti-STAT2 antibody (ab233177) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 97 kDa -
Formalin-fixed, paraffin-embedded human liver tissue stained for STAT2 using ab233177 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab233177 has been referenced in 2 publications.
- Liang Y et al. Curcumin inhibits the viability, migration and invasion of papillary thyroid cancer cells by regulating the miR-301a-3p/STAT3 axis. Exp Ther Med 22:875 (2021). PubMed: 34194553
- Cao H et al. TRIM66 promotes malignant progression of prostate carcinoma through the JAK/STAT pathway. FEBS Open Bio 10:515-524 (2020). PubMed: 31981447