Overview

  • Product name

  • Description

    Rabbit polyclonal to STAT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human STAT2 aa 801-851. The exact sequence is proprietary. NP_005410.1
    Database link: P52630

  • Positive control

    • WB: Jurkat, HEK-293T and HeLa whole cell lysates. IP: Jurkat whole cell lysate. IHC-P: Human ovarian carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab245395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 98 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
  • Sequence similarities

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications

    Tyrosine phosphorylated in response to IFN-alpha.
  • Cellular localization

    Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.
  • Information by UniProt
  • Database links

  • Alternative names

    • Homo sapiens interferon alpha induced transcriptional activator antibody
    • interferon alpha induced transcriptional activator antibody
    • ISGF 3 antibody
    • ISGF3 antibody
    • MGC59816 antibody
    • P113 antibody
    • signal transducer and activator of transcription 2 113kD antibody
    • Signal transducer and activator of transcription 2 antibody
    • STAT113 antibody
    • Stat2 antibody
    • STAT2_HUMAN antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for STAT2 using ab245395 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.

  • All lanes : Anti-STAT2 antibody (ab245395) at 0.04 µg/ml

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
    Lane 2 : Jurkat whole cell lysate at 15 µg
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 98 kDa


    Exposure time: 10 seconds
  • STAT2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245395 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245395 at 1 µg/ml.

    Lane 1: ab245395 IP in Jurkat whole cell lysate.
    Lane 2: Control IgG IP in Jurkat whole cell lysate.

    Detection: Chemiluminescence with exposure time of 3 seconds.

References

ab245395 has not yet been referenced specifically in any publications.

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