Overview

  • Product name

    Anti-STAT2 antibody - C-terminal
    See all STAT2 primary antibodies
  • Description

    Rabbit polyclonal to STAT2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human STAT2 (C terminal). The exact sequence is proprietary.
    Database link: P52630

  • Positive control

    • WB: Jurkat, Raji, K562 and THP-1 whole cell lysates. IP: HEK-293T whole cell lysate. IHC-P: Human cervical carcinoma and endometrial carcinoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab227312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/100 - 1/500.
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 97 kDa.

Target

  • Function

    Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
  • Sequence similarities

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications

    Tyrosine phosphorylated in response to IFN-alpha.
  • Cellular localization

    Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.
  • Information by UniProt
  • Database links

  • Alternative names

    • Homo sapiens interferon alpha induced transcriptional activator antibody
    • interferon alpha induced transcriptional activator antibody
    • ISGF 3 antibody
    • ISGF3 antibody
    • MGC59816 antibody
    • P113 antibody
    • signal transducer and activator of transcription 2 113kD antibody
    • Signal transducer and activator of transcription 2 antibody
    • STAT113 antibody
    • Stat2 antibody
    • STAT2_HUMAN antibody
    see all

Images

  • All lanes : Anti-STAT2 antibody - C-terminal (ab227312) at 1/2000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
    Lane 4 : THP-1 (human monocytic leukemia cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 97 kDa



    5% SDS-PAGE gel.

  • STAT2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with 2 µg ab227312. Western blot was performed from the immunoprecipitate using ab227312 at 1/1000 dilution. Anti-rabbit IgG was used as secondary antibody.

    Lane 1: HEK-293T whole cell lysate 40ug.

    Lane 2: Control IP in HEK-293T whole cell lysate with 2 μg of preimmune rabbit IgG. 

    Lane 3: ab227312 IP in HEK-293T whole cell lysate.

    7.5% SDS-PAGE gel.

  • Paraffin-embedded human cervical carccinoma tissue stained for STAT2 using ab227312 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded human endrometrial carccinoma tissue stained for STAT2 using ab227312 at 1/500 dilution in immunohistochemical analysis.

References

ab227312 has not yet been referenced specifically in any publications.

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