Product nameAnti-STAT2 antibody - C-terminal
See all STAT2 primary antibodies
DescriptionRabbit polyclonal to STAT2 - C-terminal
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey
Recombinant fragment within Human STAT2 (C terminal). The exact sequence is proprietary.
Database link: P52630
- WB: Jurkat, Raji, K562 and THP-1 whole cell lysates. IP: HEK-293T whole cell lysate. IHC-P: Human cervical carcinoma and endometrial carcinoma tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/100 - 1/500.|
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 97 kDa.|
FunctionSignal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
Sequence similaritiesBelongs to the transcription factor STAT family.
Contains 1 SH2 domain.
modificationsTyrosine phosphorylated in response to IFN-alpha.
Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.
- Information by UniProt
- Homo sapiens interferon alpha induced transcriptional activator antibody
- interferon alpha induced transcriptional activator antibody
- ISGF 3 antibody
All lanes : Anti-STAT2 antibody - C-terminal (ab227312) at 1/2000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 4 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 97 kDa
5% SDS-PAGE gel.
STAT2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with 2 µg ab227312. Western blot was performed from the immunoprecipitate using ab227312 at 1/1000 dilution. Anti-rabbit IgG was used as secondary antibody.
Lane 1: HEK-293T whole cell lysate 40ug.
Lane 2: Control IP in HEK-293T whole cell lysate with 2 μg of preimmune rabbit IgG.
Lane 3: ab227312 IP in HEK-293T whole cell lysate.
7.5% SDS-PAGE gel.
Paraffin-embedded human cervical carccinoma tissue stained for STAT2 using ab227312 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human endrometrial carccinoma tissue stained for STAT2 using ab227312 at 1/500 dilution in immunohistochemical analysis.
ab227312 has not yet been referenced specifically in any publications.