Overview

  • Product name

    Anti-STAT2 antibody [EP1814Y]
    See all STAT2 primary antibodies
  • Description

    Rabbit monoclonal [EP1814Y] to STAT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse STAT2 aa 850-950 (C terminal). The exact sequence is proprietary.

  • Positive control

    • NIH 3T3 cell lysate
  • General notes

    Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab134192 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 105 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
    • Sequence similarities

      Belongs to the transcription factor STAT family.
      Contains 1 SH2 domain.
    • Post-translational
      modifications

      Tyrosine phosphorylated in response to IFN-alpha.
    • Cellular localization

      Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.
    • Information by UniProt
    • Database links

    • Alternative names

      • Homo sapiens interferon alpha induced transcriptional activator antibody
      • interferon alpha induced transcriptional activator antibody
      • ISGF 3 antibody
      • ISGF3 antibody
      • MGC59816 antibody
      • P113 antibody
      • signal transducer and activator of transcription 2 113kD antibody
      • Signal transducer and activator of transcription 2 antibody
      • STAT113 antibody
      • Stat2 antibody
      • STAT2_HUMAN antibody
      see all

    Images

    • Anti-STAT2 antibody [EP1814Y] (ab134192) at 1/2000 dilution + NIH 3T3 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 105 kDa

    References

    This product has been referenced in:

    • Liu H  et al. microRNA-203 promotes proliferation, differentiation, and migration of osteoblasts by upregulation of Msh homeobox 2. J Cell Physiol N/A:N/A (2019). Read more (PubMed: 30854680) »
    • Yang J  et al. Combination of IFITM1 knockdown and radiotherapy inhibits the growth of oral cancer. Cancer Sci 109:3115-3128 (2018). Read more (PubMed: 29770536) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134192.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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