Product nameAnti-STAT2 antibody [Y141]
See all STAT2 primary antibodies
DescriptionRabbit monoclonal [Y141] to STAT2
SpecificityThis antibody detects both long and short forms of STAT2.
Tested applicationsSuitable for: WB, ICC, IHC-Pmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human STAT2 (N terminal). The exact sequence is proprietary.
- K562 cell lysate and human thyroid cancer
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab32367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/10000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).|
|ICC||1/50 - 1/100.|
|IHC-P||1/100 - 1/250.|
FunctionSignal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
Sequence similaritiesBelongs to the transcription factor STAT family.
Contains 1 SH2 domain.
modificationsTyrosine phosphorylated in response to IFN-alpha.
Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.
- Information by UniProt
- Homo sapiens interferon alpha induced transcriptional activator antibody
- interferon alpha induced transcriptional activator antibody
- ISGF 3 antibody
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: STAT2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: K562 whole cell lysate (20 µg)
Lane 4: THP1 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32367 observed at 97 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab32367 was shown to recognize STAT2 in wild-type HAP1 cells as signal was lost at the expected MW in STAT2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and STAT2 knockout samples were subjected to SDS-PAGE. Ab32367 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Anti-STAT2 antibody [Y141] (ab32367) at 1/500 dilution + K562 cell lysate
Predicted band size: 98 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded human thyroid cancer using ab32367
ab32367 has not yet been referenced specifically in any publications.