• Product name

    Anti-STAT2 (phospho Y690) antibody
    See all STAT2 primary antibodies
  • Description

    Rabbit polyclonal to STAT2 (phospho Y690)
  • Host species

  • Specificity

    ab53132 detects endogenous levels of STAT2 only when phosphorylated at tyrosine 690.
  • Tested applications

    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic phosphopeptide derived from human STAT2 around the phosphorylation site of tyrosine 690 (R-K-YP-L-K).

  • Positive control

    • WB: HeLa cell extracts IHC-P: Human lung carcinoma tissue



Our Abpromise guarantee covers the use of ab53132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.


  • Function

    Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
  • Sequence similarities

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational

    Tyrosine phosphorylated in response to IFN-alpha.
  • Cellular localization

    Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.
  • Information by UniProt
  • Database links

  • Alternative names

    • Homo sapiens interferon alpha induced transcriptional activator antibody
    • interferon alpha induced transcriptional activator antibody
    • ISGF 3 antibody
    • ISGF3 antibody
    • MGC59816 antibody
    • P113 antibody
    • signal transducer and activator of transcription 2 113kD antibody
    • Signal transducer and activator of transcription 2 antibody
    • STAT113 antibody
    • Stat2 antibody
    • STAT2_HUMAN antibody
    see all


  • All lanes : Anti-STAT2 (phospho Y690) antibody (ab53132) at 1/300 dilution

    Lane 1 : HeLa cell extract treated with IFN (2500U/ml, 30mins)
    Lane 2 : HeLa cell extract treated with IFN (2500U/ml, 30mins) with immunising peptide

    Predicted band size: 98 kDa
    Observed band size: 98 kDa

  • ab53132, at 1/50 dilution, staining STAT2 in paraffin embedded human lung carcinoma tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.


This product has been referenced in:

  • Liu H  et al. microRNA-203 promotes proliferation, differentiation, and migration of osteoblasts by upregulation of Msh homeobox 2. J Cell Physiol N/A:N/A (2019). Read more (PubMed: 30854680) »
  • Guan G  et al. Down-regulation of IFIT3 protects liver from ischemia-reperfusion injury. Int Immunopharmacol 60:170-178 (2018). WB ; Mouse . Read more (PubMed: 29734133) »
See all 16 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A


Unfortunately, the blocking peptide for ab53132 is not available. In order to assist in your determing what the identity of your two bands are, I did some research and found that IFN-alpha and IFN-beta induce activation of Jak kinases, which lead to phosphorylation of STAT1 and STAT2. I found a nuclear lysate (STAT2 is translocated to the nucleus upon phosphorylation/activation by IFN alpha) in our catalog of U937 cells that were treated with IFN alpha. The catalog number of this antibody is ab14907:


Alternatively, you can treat your own cells with IFN alpha as a positive control, and perhaps perform a nuclear fractionation of your samples to determine the identity of the two bands since phosphorylated STAT2 should be present in the nucleus. There is also the possibility that you are detecting both isoforms and they are somehow separating more than expected in the gel. A link to the uniprot file for STAT 2 depicting both isoforms is given below:


Read More
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citric acid (pH 6.0) for 10 min at ~100¯C
Mouse Tissue sections (Brain)
Yes - 0.02% triton

Mr. Shachar Dagan

Verified customer

Submitted Mar 10 2014


Thank you fory our message which has been forwarded to me as my colleague ##### is away from the office today.

I am sorry I am not able to trace any previous correspondence from the email address you have used, and so I hope I can provide adequate information for you.

The concentrations and amounts can vary for each antibody, so I can recommend to review individual online datasheets for information. I can confirm the follownig concentrations for you:

STAT1 (ab2415)
We sell 1 ml at 0.2mg/ml
https://www.abcam.com/index.html?datasheet=2415 (or use the following: https://www.abcam.com/index.html?datasheet=2415).

pSTAT2 (ab53132)
We sell 100 ug at 1mg/ml (100 ul)
https://www.abcam.com/index.html?datasheet=53132 (or use the following: https://www.abcam.com/index.html?datasheet=53132).

STAT2 (ab53149)
We sell 100 ug at 1mg/ml (100 ul)
https://www.abcam.com/index.html?datasheet=53149 (or use the following: https://www.abcam.com/index.html?datasheet=53149).

pSTAT1 (ab30645)
We sell 100 ug at 1mg/ml (100 ul)
https://www.abcam.com/index.html?datasheet=30645 (or use the following: https://www.abcam.com/index.html?datasheet=30645).

I hope this will be helpful. If you have any further questions, please do not hesitate to contact me.

Read More


Thank you for your enquiry.

Here is the link for the activated Stat1 reference we discussed:


It uses the following antibody, ab4742:


I hope this is helpful. Please contact me again with any further questions.

Read More


I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab111609.

As we discussed, this antibody is backordered 2-3 weeks. To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

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