Overview

  • Product name

    Anti-STAT2 (phospho Y690) antibody
    See all STAT2 primary antibodies
  • Description

    Rabbit polyclonal to STAT2 (phospho Y690)
  • Host species

    Rabbit
  • Specificity

    ab53132 detects endogenous levels of STAT2 only when phosphorylated at tyrosine 690.
  • Tested applications

    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic phosphopeptide derived from human STAT2 around the phosphorylation site of tyrosine 690 (R-K-YP-L-K).

  • Positive control

    • WB: HeLa cell extracts IHC-P: Human lung carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab53132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

Target

  • Function

    Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
  • Sequence similarities

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications

    Tyrosine phosphorylated in response to IFN-alpha.
  • Cellular localization

    Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.
  • Information by UniProt
  • Database links

  • Alternative names

    • Homo sapiens interferon alpha induced transcriptional activator antibody
    • interferon alpha induced transcriptional activator antibody
    • ISGF 3 antibody
    • ISGF3 antibody
    • MGC59816 antibody
    • P113 antibody
    • signal transducer and activator of transcription 2 113kD antibody
    • Signal transducer and activator of transcription 2 antibody
    • STAT113 antibody
    • Stat2 antibody
    • STAT2_HUMAN antibody
    see all

Images

  • All lanes : Anti-STAT2 (phospho Y690) antibody (ab53132) at 1/300 dilution

    Lane 1 : HeLa cell extract treated with IFN (2500U/ml, 30mins)
    Lane 2 : HeLa cell extract treated with IFN (2500U/ml, 30mins) with immunising peptide

    Predicted band size: 98 kDa
    Observed band size: 98 kDa

  • ab53132, at 1/50 dilution, staining STAT2 in paraffin embedded human lung carcinoma tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.

References

This product has been referenced in:

  • Liu H  et al. microRNA-203 promotes proliferation, differentiation, and migration of osteoblasts by upregulation of Msh homeobox 2. J Cell Physiol N/A:N/A (2019). Read more (PubMed: 30854680) »
  • Guan G  et al. Down-regulation of IFIT3 protects liver from ischemia-reperfusion injury. Int Immunopharmacol 60:170-178 (2018). WB ; Mouse . Read more (PubMed: 29734133) »
See all 16 Publications for this product

Customer reviews and Q&As

Filter by Application

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citric acid (pH 6.0) for 10 min at ~100¯C
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.02% triton
Fixative
Paraformaldehyde

Mr. Shachar Dagan

Verified customer

Submitted Mar 10 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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